摘要
用 CTAB/ DOPE脂质体包裹新城疫病毒血凝素神经氨酸酶 ( HN)基因的真核表达质粒 pc HNM免疫 5周龄仔鸡 ,1周后免疫鸡血清中的特异性 HI抗体开始升高 ,至第 6周空白对照组抗体 2 .0 6 ,裸 DNA免疫鸡 4.0 9,脂质体 DNA免疫组为 5 .2 0 ;此时用新城疫强毒 F48E9攻击 ,未免疫组鸡的存活率为 2 7.2 8% ,裸 DNA免疫组 81 .82 % ,CTAB脂质体 DNA免疫组 1 0 0 .0 0 % ,表明该质粒 DNA被脂质体包裹后 ,不仅增加了动物的特异性抗体产生能力 。
Recombinant plasmid pcHNM DNA of newcastle virus HN gene was encapsulated in CTAB/DOPE liposomes. Five week old chickens were intramuscularly inoculated with 500 mg/L of the liposome entrapped pcHNM DNA. One week after vaccination, HI antibody titers started to increase and maintained up to 6 th week. The antibody titer of the control group was 2.30, naked DNA group 4.09,CTAB/DOPE liposome DNA group 5.20, Six weeks after inoculation, the chickens were challenged with 0.5 mL of 10 -4 F48E9 new castle disease virus. The livability of the control group was 27.28%, naked DNA group 81.82%,CTAB liposome DNA group 100.00%. The result showed that inoculating the liposome encapsulated plasmid DNA was more effective in stimulating the special humor responses and cellular responses than that of the naked DNA,and were more efficient to protect the chickens from the lethal NDV challenge.
出处
《动物医学进展》
CSCD
2001年第1期41-43,共3页
Progress In Veterinary Medicine
基金
国家"九五"攻关项目! (96 - 0 0 5 - 0 2 - 0 4- 0 4)
