摘要
目的：探讨胎盘儿茶酚氧位甲基转移酶（COMT）基因rs4680、rs6269位点单核苷酸多态性（SNP）与子痫前期发病的关系。方法：选择2011年3月至2012年8月在南方医科大学附属深圳市妇幼保健院产科住院分娩的孕妇217例，其中子痫前期孕妇92例为子痫前期组，健康孕妇125例为对照组，收集两组孕妇产后胎盘组织，提取胎盘DNA，应用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）平台及MassARRAY-IPLEX技术对COMT基因rs4680及rs6269位点进行检测，分析其基因型及等位基因分布情况。结果：胎盘组织COMT基因rs4680位点基因型GG、GA、AA的基因型频率和G、A等位基因频率在子痫前期组与对照组之间的分布差异均无统计学意义（P〉0.05）。两组胎盘COMT基因rs6269位点GG、GA、AA3种基因型频率差异有统计学意义（P=0.019）；且G、A等位基因频率在子痫前期组和对照组分布差异有统计学意义（X2=6.382，P=0.012），A／GOR=1.707（95％CI=1.125～2.590）。胎盘COMTrs4680与rs6269单核苷酸多态性与孕妇血压无明显相关性。结论：胎盘COMTrs6269单核苷酸多态性与子痫前期发生相关，携带A等位基因是发生子痫前期的危险因素；胎盘COMTrs4680单核苷酸多态性与子痫前期发生无关。
Objective：To investigate whether polymorphisms of placental COMT gene are associated with preeclampsia,and to explore the role of COMT in the pathogenesis of preeclampsia. Methods：92 patients with preeclampsia and 125 normal pregnant women were enrolled, Clinical characteristics and placental tissue were collected, and placental COMT（ rs4680 and rs6269）polymorphisms were genotyped by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS） and MassARRAY-IPLEX. Results ：There were no significant differences either in rs4680 genotypes or allele frequencies between patients with preeclampsia and controls （ P 〉 0.05）. There were significant differences both in rs6269 genotypes and allele frequencies between patients with preeclampsia and controls（ P = 0.019 and P =0.012, respectively）. The odds ratio for the risk of preeclampsia was 1. 707 （95% C I 1. 125 -2.590）in women with A allele. There was no association between placental COMT genotypes and blood pressure. Conclusions. Placental COMT rs6269 genotype contributes to the genetic susceptibility of preeclampsia in Hart Chinese women. Placental COMT rs6269 genotypes with A allele gene increase risk of preeclampsia. Placental COMT rs4680 genotype has no association with preeclampsia in Chinese Han women.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2014年第5期366-369,共4页
Journal of Practical Obstetrics and Gynecology
