摘要
目的观察塞来昔布环氧合酶-2(COX-2)选择性抑制剂引起糖尿病大鼠视网膜组织促红细胞生成素的改变,探讨其防治早期糖尿病视网膜的机制。方法 40只SD雄性大鼠随机分为4组,即正常组、糖尿病组、对照组、实验组,每组10只。1%链尿佐菌素腹腔注射建立糖尿病模型,饲养3个月后,处死大鼠取视网膜,HE染色观察视网膜形态学变化,SYBR荧光实时定量PCR检测视网膜组织中COX-2、EPO mRNA表达变化。结果 3个月后,HE染色糖尿病组和对照组结构出现了改变;COX-2、EPO mRNA在糖尿病组和对照组,组间两两比较,无统计学差异(P<0.05),其余各组间比较均有显著性差异(P>0.05)。结论塞来昔布能够有效促进EPO的表达,可以用于早期糖尿病视网膜病变防治。
Objective To observe the effect of cyclooxygenase-2 inhibitor on the expression of erythropoietin （ EPO） in diabetic rats retina and the effect of celecoxib on prevention of early diabetic retinopathy .Methods Forty SD male rats were randomly divided into four groups：the normal group , diabetes group , control group and experimental group , 10 in each group.Diabetes model was established by intraperitoneal injection of streptozotocin （STZ） （50 mg/kg）.All of the rats were sacrificed at 3 months.Retinae were HE stained to observe morphological changes , and SYBR real-time fluo-rescent quantitative PCR was used to detect COX-2 and EPO mRNA expression changes .Results After 3 months, HE staining showed structural changes in diabetes group and control group .There was no difference in COX-2 or EPO mRNA expression between diabetes group and the control group （ P 〈0.05）, but between diabetes group and other two groups （ P 〈0.05）.Conclusions Celecoxib could effectively promote the expression of EPO , thus can be used for early preven-tion and treatment for diabetic retinopathy .
出处
《临床眼科杂志》
2014年第2期176-179,共4页
Journal of Clinical Ophthalmology
基金
国家自然科学基金资助(编号:81102619)
福建省自然科学基金项目(编号:2011JO1197)
