摘要
在5 000 L罐中进行了井冈霉素补糖试验,结果表明补糖能提高井冈霉素的效价,通过测定不同补糖种类、补糖时间、补糖量条件下井冈霉素效价,得到优化后的补糖工艺:在发酵液中残糖为3.5%时,一次性补加终浓度为2%的淀粉液化糖。采用该工艺,较常见未补料工艺井冈霉素产量提高19.0%。
In this paper, a new sugar supplement test in the 5000L fermenter for Validamycin was developed, and the results showed sugar can improve potency of Validamycin. Different types of sugar, stages of sugar supplement, and amounts of sugar were applied to study the effect of Validamycin poteny. Finally we got most suitable process for Validamycin poteny： when the concentration of residual sugar was 3.5%, one-time top-up 2% liquefied starch sugar to satisfy the needs of fermentation. Compared to routine pattern in fermentation process, Validamycin production was increased by 19.0%.
出处
《发酵科技通讯》
CAS
2014年第1期4-5,10,共3页
Bulletin of Fermentation Science and Technology
关键词
工业化
井冈霉素
发酵
补糖
industrilization
validamycin
fermentation
carbohydrate supplement
