摘要
井冈霉素是最成功的生物源杀菌剂之一,也是防治水稻纹枯病的最常用与最有效的农药之一。文章综述了井冈霉素的理化性质及关于井冈霉素生物活性的研究,井冈霉素不仅抑制海藻糖酶活性,而且还具有抑制真菌的纤维素降解酶、多聚半乳糖醛酸酶和肌醇生物合成的活性;总结了井冈霉素的检测方法,通过色谱与质谱联用等方法来完成,最低检测量达到了0.4 ng。提出井冈霉素的生物合成是先将葡萄糖经过戊糖磷酸途径生成景天庚酮糖-7-磷酸,然后将葡萄糖与景天庚酮糖-7-磷酸经过10个酶催化,最终合成了井冈霉素A。最后评价了井冈霉素的发酵生产工艺,经过培养基组分优化、补料添加、发酵条件控制与分离工艺改进,井冈霉素的生产工艺也日趋完善。
Jinggangmycin is one of the most successful bio-pesticides and is mainly used to protect Oryza sativa against Rhizoctonia solani. This paper reviewed the physicochemical property of jinggangmycin and theresearches about the bioactivity of Jinggangmycin. jinggangmycin not only inhibited the activity of trehalase,cellulose- degrading enzymes and polygalacturonase but also affected the biosynthesis of inositol. Then theauthor summed up the detection method of Jinggangmycin. The lowest limit of jinggangmycin that could bedetected was 0.4 ng with the LC-MS or GC-MS method. Then biosynthesis pathway of jinggangmycin was putforward, which was producing sedoheptulose-7-phosphate using glucose by pentose phosphate pathway andthen catalyzing glucose and sedoheptulose- 7- phosphate by 10 enzymes. The manufacturing technique ofjinggangmycin has been greatly improved with the optimization of media components and feed- batch, thecontrol of fermentation conditions and the separation process.
出处
《中国农学通报》
2015年第22期191-198,共8页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金"北五味子内生真菌及其木质素类化合物的研究"(31100036)
"小兴安岭阔叶红松林生态系统对N沉降增加的影响"(31170421)
"大兴安岭北方森林生态系统碳循环对模拟N沉降的响应"(31370494)
黑龙江大学实验室创新项目
关键词
井冈霉素
有效霉素
生物农药
吸水链霉菌
水稻纹枯病
jinggangmycin
validamycin
bio-pesticide
Streptomyces hygroscopicus
rice sheath blight
