摘要
目的:研究N-乙酰半胱氨酸(NAC)对噪声性听力损伤的保护作用。方法:32只雄性白色豚鼠随机分成4组,每组8只。从噪声暴露前7天(7d)开始,分别灌胃给予蒸馏水、NAC 70、NAC 100、NAC 140mg/kg,1次/d,连续14d。在用药第8天(d 8),各组动物在中心频率4kHz、强度115dB(A)的倍频程噪声暴露4h,建立暴噪模型。测定噪声暴露前、噪声暴露后d 3、d 8、d 11各组豚鼠脑干听觉反应(ABR)的阈值。以听阈偏移反映听力受损情况,组织学检查耳蜗内外毛细胞的损伤程度,来评价NAC对豚鼠噪声性听力损伤的保护作用。结果:灌胃给予NAC 100、NAC 140mg/kg可在一定程度上减少噪声暴露后d 3、d 8、d 11豚鼠ABR的阈移值,减轻毛细胞的损伤。结论:NAC对噪声引起的急性和亚急性听力损伤均具有一定的保护作用。
Objective： To study the protective effect of N-acetylcysteine（NAC）on noise induced hearing loss （NIHL）. Methods： Thirty-two male white guinea pigs were randomly divided into 4 groups, each consisting of 8 animals. Seven days before noise exposure,guinea pigs were administered distilled water （control）, NAC 70, NAC 100 and NAC 1.40 mg/kg by gavage once daily for 14 days. On the 8th day（d 8）, animals of the 4 groups were exposed to the noise of 4 kHz center frequency, 115 dB sound pressure level （SPL） for 4 hours to develop noise exposure model. The thresholds of auditory brainstem response （ABR） before noise exposure and 3, 8 and 11 days after the exposure were determined in the guinea pigs of the 4 groups. The damage extent of inner and outer hair cells of cochlea was evaluated histologically by scanning electron micro- scope. The protective effects of NAC on NIHL were evaluated by comparing the threshold shifts of A13R and the damage extent of bair cells between the ,1 groups. Results： The threshold shifts of ABR at days 3, 8 and 11 after noise exposure and damage of hair cells induced hy noise exposure could be decreased to some extent with the treatment of NAC 100, NAC 140 mg/kg. Conclusion： NAC has some protective effects on noise induced acute and sub-acute hearing loss.
出处
《药学服务与研究》
CAS
CSCD
2014年第2期107-110,共4页
Pharmaceutical Care and Research
基金
国家科技重大专项重大新药创制资助项目(2011ZXJ09106-07B)
