摘要
目的:研究短刺海马的化学成分。方法:采用乙醇提取和硅胶柱色谱、Sephadex LH-20柱色谱等手段对样品进行分离纯化,再通过波谱分析并结合文献对照,鉴定化合物的结构。结果:从短刺海马乙醇提取物中分离得到9个化合物,经鉴定为胆甾醇(1)、胆甾-4-烯-3-酮(2)、3β,5α,9α-三羟基-(22E,24R)-麦角甾-7,22-二烯-6-酮(3)、24-甲基-5α-胆甾-7,22-二烯-3β,5,6β-三醇(4)、3β-羟基-7-甲氧基-胆甾-5-烯(5)、3β-羟基-胆甾-5-烯-7-酮(6)、肌酸酐(7)、鸟嘧啶(8)、腺嘌呤(9)。结论:9个化合物均为首次从短刺海马中分离得到。该结果可为短刺海马的合理利用和进一步产品开发奠定物质基础。
OBJECTIVE： To study the chemical constituents of Hippocampus erinaceus. METHODS： After extracted with etha- nol, H. erinaceus was purified by silica gel column and Sephadex LH-20 column chromatography, etc. The structures of the isolat- ed compounds were identified by spectral analysis and literature search. RESULTS： 9 compounds were isolated and identified from the ethanol extract ofH, erinaceus： such as cholesterol （1）, cholest-4-en-3-one （2）, 3β, 5 α, 9α-trihydroxy-（22E, 24R）-ergosta-7, 22-dien-6-one （3）, 24-methyl-5 α-cholesta-7,22-diene-3β, 5,6β-triol （4）, 3β-hydroxy-7-methoxy-cholesta-5-en （5）, 3β-hydroxycho- lest-5-en-7-one （6）, creatinine （7）, uracil （8）, and adenine （9）. CONCLUSIONS： All of the compounds are isolated from H. eri- naceus for the first time. The results can lay the material foundation for rational utilization and further development of the products of H. erinaceus.
出处
《中国药房》
CAS
CSCD
2014年第19期1780-1782,共3页
China Pharmacy
关键词
短刺海马
化学成分
甾醇
波谱分析
Hippocampus erinaceus
Chemical constituents
Sterol
Spectral analysis
