摘要
为从豆豉中获得产γ-氨基丁酸的乳酸菌,采用高效液相色谱法对从广西黄姚豆豉中分离筛选得到的16株乳酸菌的产γ-氨基丁酸能力进行了研究。结果表明:菌株HY15的产γ-氨基丁酸能力较好。该菌株在含1%(W/W)谷氨酸钠的MRS发酵培养基中37℃厌氧培养48h后,发酵液中γ-氨基丁酸产量达到0.161g/L。经形态学特征、生理生化试验和16SrDNA基因鉴定,确定菌株HY15为戊糖片球菌(Pediococcus pentosaceus)。
In order to obtainγ-aminobutyric acid from lactic acid bacteria,the ability ofγ-aminobutyric acid production of the 16 strains of lactic acid bacteria isolated from Huangyao Douchi,Guangxi Province are investigated by using High Performance Liquid Chromatography.The results show that the strain HY15 has better producing ability ofγ-aminobutyric acid.After 48 h of inoculation in MRS medium containing 1% （W/W）monosodium glutamate,it can produceγ-aminobutyric acid with the concentration of 0.161 g/L.Based on morphological characteristics,physiological and biochemical tests, and 16S rDNA sequence analysis,the strain HY15 is identified as Pediococcus pentosaceus.
出处
《中国调味品》
CAS
北大核心
2014年第5期53-58,共6页
China Condiment
关键词
Γ-氨基丁酸
乳酸菌
筛选
鉴定
γ-aminobutyric acid
lactic acid bacteria
screening
identification
