摘要
目的 掌握猕猴耳廓软骨细胞的体外分离、培养和鉴定技术 ,探讨耳廓软骨作为软骨细胞供区的可行性。材料与方法 :对 6只猕猴进行耳廓软骨取材、软骨细胞的分离 ,并行单层贴壁培养。通过倒置显微镜观察细胞生长情况并行细胞生长曲线的绘制 ;通过免疫组织化学染色对细胞分泌的基质成分进行鉴定。结果 :6只猕猴的耳廓软骨经分离后 ,获得了高纯度、高活性的软骨细胞 ,并成功地进行了体外培养 ;软骨细胞倍增时间为 98小时 ;免疫组织化学染色发现体外培养的软骨细胞具有分泌胶原基质的能力 ,但第三代细胞分泌Ⅰ、Ⅱ型胶原的能力无明显区别。结论 :利用猕猴耳廓软骨细胞体外分离及培养 ,能成功地获得具有体内活性的软骨细胞 。
Objective:To culture the auricular chondrocytes of rhesus monkey in vitro,and to certify the possibility of auricular cartilage as an ideal donor site for chondrocytes transplantation.Methods:The auricular cartilages of 6 rhesus monkeys were dissected and digested,the chondrocytes were isolated and cultured in F 12 medium.The changes of cellular morphology were investigated with inverted microscope.The cellular activities were studied with immunohistochemistry(IHC).Results:The homogenous,high activity chondrocytes were harvested and cultured iv vitro successfully and IHC showed that there was no significant difference between type Ⅰ and type Ⅱ collagen stain in 3rd generation.Conclusions:Auricular cartilage of rhesus monkey is an ideal donor site for chondrocytes transplantation. [
出处
《口腔医学纵横》
CSCD
2001年第1期28-30,共3页
Journal of Comprehensive Stomatology
关键词
猕猴
耳廊软骨
细胞培养
免疫组织化学
体外培养
鉴定
Rhesus monkey Auricular cartilage Chondrocyte cultivation Immunohistochemistry
