摘要
目的:探讨长期游泳运动对2型糖尿病大鼠周围神经病变的影响及可能机制。方法:雄性Wistar大鼠高糖高脂饲料喂养联合链脲佐菌素注射制备成T2DM大鼠模型后,随机分为空白对照组(C组)、单纯运动组(CE组)、糖尿病对照组(DM组)和糖尿病运动组(DME组)。CE组和DME组进行8周游泳训练(6 d/week),第1周前3 d练习时间分别为20、30和45 min,第4天起每天持续游泳60 min。运动8周后测定各组大鼠坐骨神经的传导速度(MNCV),以及该组织中肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、C反应蛋白(CRP)的含量,观察其形态结构。结果:实验末,与DM组相比,DME组的MNCV明显提高(P<0.05),坐骨神经组织中TNF-α、IL-6和CRP水平均有所降低,但差异无统计学意义(P>0.05)。DM组坐骨神经光镜下可见明显的损伤,经运动干预后病变程度减轻。结论:8周游泳运动可提高MNCV,减轻DM造成的神经损伤,对周围神经起保护作用,其机制可能与减轻炎症反应有关。
AIM ： To explore the influence of long-term swimming on peripheral neuropathy in type 2 diabetic rats. METHODS ： Male Wistar rats were fed with a high-fat and high-fructose diet, and injected with streptozocin to estab- lish a model of type 2 diabetes mellitus. The rats were randomly divided into 4 groups ： blank control group （ C group）, ex- ercise control group （ CE group）, diabetes mellitus group （ DM group） and diabetes mellitus ＋ exercise group （ DME group）. The rats in CE group and DME group received 8-week swimming training （6 d/week）. The training time was 20, 30 and 45 rain in the first 3 d,respeetively, and then it increased to 60 rain a day. Eight weeks later, the motor nerve conduc- tion velocity （MNCV） and the levels of tumor necrosis factor ct （ TNF-ct）, interleukin 6 （ IL-6 ） and C-reactive protein （CRP） in sciatic nerve tissues of the rats were measured. The morphological changes of the sciatic nerve were also observed under light microscope. RESULTS： Compared with DM group, 8-week swimming obviously accelerated the MNCV （P 〈 0. 05）, decreased the levels of TNF-ot, IL-6 and CRP in DME group （ but no significant difference, P 〉 0.05）. The obvi- ous nerve injury in DM group was observed. However, the pathological change of the sciatic nerve in DME group was re- lieved. CONCLUSION： Eight-week swimming training significantly accelerates the MNCV, attenuates the nerve injury in diabetic rats and has protective effect on peripheral nerve, which may be correlated with relieving the inflammatory reaction.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2014年第4期719-724,共6页
Chinese Journal of Pathophysiology
