摘要
实验研究发现淫羊藿对不同类型骨质疏松大鼠模型均有骨代谢调节作用,临床研究亦发现采用淫羊藿治疗绝经后骨质疏松症也取得了明显的效果。其药理作用机制可能与降低破骨细胞内Ca2+浓度,影响成骨细胞增殖、分化、矿化;增加骨护骨素(OPG)mRNA的表达,提高成骨细胞Osterix基因表达水平,从而促进骨形成;通过升高人成骨细胞骨形成蛋白-2(BMP-2)mRNA的表达,增加骨形态生成蛋白2的生成刺激人类成骨细胞的增殖和分化;通过抑制TNF-αmRNA和促进TGF-β1mRNA的表达促进转化生长因子(TGF)-β1的产生,抑制肿瘤坏死因子-α(TNF-α)表达,进而阻止骨髓基质中破骨细胞的生成,减少骨质丢失;淫羊藿总黄酮增加骨桥蛋白(OPN)mRNA和Ⅰ型胶原的表达促进骨的生成;淫羊藿苷可通过选择性上调下丘脑和海马ERα,ERβmRNA的表达,降低骨组织中IL-6的表达,从而减少骨吸收;淫羊藿总黄酮可通过抑制DKK1蛋白的表达,调控去势雌性大鼠BMSCs成骨和成脂分化平衡调节骨代谢;淫羊藿苷通过上调Cbfal、BMP2和BMP4mRNA的表达而促进成骨细胞的分化,诱导大鼠骨髓间充质干细胞向成骨细胞分化;淫羊藿总黄酮可显著改善雄性生殖系统和生殖内分泌功能,促进性腺的分泌,产生类雄激素样作用而促进成骨细胞的增殖与分化。笔者从多个层面对淫羊藿黄酮类成分抗骨质疏松作用机制进行阐述,提出今后在此研究基础上尚需进一步深入全面、系统的研究和阐述。
Researches have found that the regulating effect of epimedium on bone metabolism exists in different osteoporotic rat models. The clinical studies also indicate an obvious efficacy of epimedium in the treatment of postmenopausal osteoporosis. Its possible mechanism may rely on reducing Ca2~ level in osteoclasts, influencing the proliferation, differentiation, and mineralization of osteoblasts. It may also increase the expression of OPG mRNA and promote the expression of Osterix gene in osteoblasts, thus promoting bone formation. Epimedium can also promote the expression of BMP-2 mRNA, increase the expression of BMP-2, in order to stimulate the proliferation and differentiation of osteoblasts. Meanwhile, by inhibiting the expression of TNF-a mRNA and promoting the expression of TGF-~I mRNA, epimedium promotes the production of TGF-131, thus preventing the generation of osteoclasts in bone marrow and reducing bone loss. Total epimedium flavonoids （TFP） can promote bone formation by increasing the expression of OPN mRNA and type-I collagen. By selectively up-regulating the expression of ERa and ERI3 mRNA in the hypothalamus and the hippocampus, epimedium can down-regulate the expression of IL-6, thus reducing the bone resorption. TFP can regulate bone metabolism by inhibiting the expression of DKK1 protein, thus controlling the balance of osteogeic and adipogenic differentiation of BMSCs in OVX rats. Epimedium can promote the expression of Cbfal, BMP2, and BMP4 mRNA, thus promoting the differentiation of osteoblasts and inducing the osteogenic differentiation of rat BMSCs. TFP can remarkably improve the function of male genital system and reproductive endocrine, stimulate the secretion of gonad, and exert.androgen-like function to accelerate the proliferation and differentiation of osteoblasts. This article elaborates the mechanism of epimedium flavonoids in anti-osteoporosistreatment from several aspects, proposing a deeper and systemic research and description in the future.
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2014年第4期452-456,共5页
Chinese Journal of Osteoporosis
关键词
淫羊藿总黄酮
骨代谢
药理机制
Total epimedium flavonoids
Bone metabolism
Pharmacological mechanism
