摘要
目的观察氟铝单独及联合对小鼠成骨样细胞MC3T3-E1细胞增殖、分化及Runx2、Osterix mRNA表达的影响。方法在细胞培养液中加入50μmol/L氟化钠或/和5μmol/L氯化铝,观察MC3T3-E1细胞增殖和分化能力,用RT-PCR法测定MC3T3-E1细胞Runx2和Osterix mRNA表达的情况。结果与对照组相比,氟铝联合染毒可显著提高MC3T3-E1细胞增殖和分化能力(P<0.05),MC3T3-E1细胞Runx2和OsterixmRNA的表达水平也显著提高(P<0.05),且氟铝具有协同作用(P<0.05)。结论氟铝联合通过刺激成骨细胞增殖、分化,提高成骨细胞Runx2和Osterix基因表达水平,从而促进骨形成。
Objective To study the combined effect of fluoride and aluminum on cell proliferation and differentiation as well as on the expression of Runx2 and Osterix mRNA in osteoblastic cell line.Methods MC3T3-E1 cells were cultured in medium containing 50μmol/L NaF or/and 5μmol/L AlCl3.Cell proliferation and differentiation was examined and the expression level of Runx2 and Osterix mRNA were determined by RT-PCR.Results Compared with the control group,cell proliferation and differentiation and the expression of Runx2 and Osterix mRNA in MC3T3-E1 cells were enhanced by fluoride and aluminum（P 0.05）.There was a synergistic effect of fluoride and aluminum on the expression of Runx2 and Osterix mRNA in MC3T3-E1 cells.Conclusion Bone formation was promoted by fluoride and aluminum in stimulating the proliferation and differentiation of osteoblastic cells and the expression of Runx2 and Osterix mRNA in the cells.
出处
《卫生研究》
CAS
CSCD
北大核心
2011年第2期164-166,共3页
Journal of Hygiene Research
基金
国家自然科学基金项目(No.30600509)
