摘要
目的优化菲牛蛭Poecilobdella manillensis的人工饲料3种主要成分配比,确定饲料的最佳配方。方法采用湿土网箱法养殖菲牛蛭,以存活率和个体体质量增长率为考察指标,采用L9(34)正交试验法,考察血球蛋白粉(NP-90)、血浆蛋白粉(NP-2002)和维生素C(VC)3个因素,优选菲牛蛭人工饲料。采用层次分析法统计正交试验,分析各因素各水平对试验结果的影响权重。结果以菲牛蛭存活率为考察指标时,3种因素对成活率的影响为VC>NP-90>NP-2002,以0NP-90、1%NP-2002和0.05%VC组合为最佳;以菲牛蛭个体体质量增长率为考察指标时,3种因素对个体体质量增长率的影响大小依次为NP-2002>NP-90>VC,饲料配方以0.5%NP-90、3%NP-2002和0.05%VC组合为最优。层次分析表明,各因素对正交试验的指标值影响的主次顺序为NP-90>NP-2002>VC,0.5%NP-90、3%NP-2002和0.05%VC的权重最大,正交试验的最优方案为0.5%NP-90、3%NP-2002和0.05%VC。按0.5%NP-90、3%NP-2002和0.05%VC及其他成分配制饲料饲喂菲牛蛭,进行验证试验,结果饲料组菲牛蛭成活率达100%,个体体质量增长率达19.91%。结论按0.5%NP-90、3%NP-2002和0.05%VC及其他成分配制饲料可作为菲牛蛭人工饲料。
Objective To optimize the formula of artificial diets for Poecilobdella manillens＆. Methods Survival rate and individual body weight growth rate of P rnanillensis were chosen as indexes. The three factors [NP-90 spray-dried hemoglobin powder, NP-2002 spray-dried plasma powder, and vitamin C （VC）] of the formula of artificial diets were investigated by L9（34） orthogonal test. AHP was applied to statistical analysis on non-interacting orthogonal test, the effect weight of each factor for test result was obtained, and principal-secondary sequence of factors and optimal project was given. Results The effects of the three components in artificial diets on the survival rate were in the following decreasing order： VC〉NP-90〉NP-2002. And the optimization of components of artificial diets was 0 NP-90 spray-dried hemoglobin powder, 1% NP-2002 spray-dried plasma powder, and 0.05% VC. When the individual body weight growth rate was chosen as the index, the effects of the three components in artificial diets on the individual body weight growth rate were in the following decreasing order： NP-2002〉NP-90〉VC. The optimization of components of artificial diets was 0.5% NP-90 spray-dried hemoglobin powder, 3% NP-2002 spray-dried plasma powder, and 0.05% VC. The weight of principal- secondary sequence of factors and levels influences on the tests＇ results was NP-90 〉NP-2002 〉 VC. The weight of 0.5% NP-90 spray-dried hemoglobin powder, 3% NP-2002 spray-dried plasma powder and 0.05% VC was maximum. And the optimization of components in artificial diets was 0.5% NP-90 spray-dried hemoglobin powder, 3% NP-2002 spray-dried plasma powder, and 0.05% VC. According to the results, P. manillensis bred with the artificial diets （0.5% NP-90 spray-dried hemoglobin powder, 3% NP-2002 spray-dried plasma powder, 3% NP-2002 spray-dried plasma powder, 0.05% VC, and other components） had the survival rate of 100% and the individual body weight growth rate was 19.91%. Conclusion The optimal artificial diets could be used
出处
《中草药》
CAS
CSCD
北大核心
2014年第6期782-785,共4页
Chinese Traditional and Herbal Drugs
基金
辽宁省海洋与渔业厅"吸血水蛭工厂化高密度养殖技术研究"项目(200903)
关键词
菲牛蛭
人工饲料
正交试验
湿土网箱法
存活率
层次分析法
血球蛋白粉
血浆蛋白粉
维生素C
Poecilobdella manillensis Lesson
artificial diets
orthogonal test
cage culture with moist earth
survival rate
analytic hierarchy process
NP-90 spray-dried hemoglobin powder
NP-2002 spray-dried plasma powder
vitamin C
