摘要
目的:考察牛蒡苷元对H89诱导的人神经母细胞瘤SH-SY5Y细胞损伤的保护作用。方法:用蛋白激酶A抑制剂H89处理SH-SY5Y细胞,建立细胞损伤模型。将SH-SY5Y细胞分成正常组(正常细胞)、模型组(经H89处理的细胞)、H89+牛蒡苷元组(经H89处理后给予牛蒡苷元处理的细胞)和H89+丹酚酸B组(阳性对照组,经H89处理后给予丹酚酸B处理的细胞)。采用MTT法检测细胞活力、免疫荧光细胞化学法检测细胞中β-淀粉样肽和神经营养因子-3的表达以及Hoechst33258染色法检测细胞凋亡率。结果:H89诱导的细胞损伤模型造模成功。牛蒡苷元在低浓度(0.5μmol·L-1)时对细胞损伤模型的保护作用最佳,与模型组相比,H89+低浓度牛蒡苷元组细胞的细胞活力显著提高(P<0.01),细胞中β-淀粉样肽的表达下调5.17%(P<0.05),而神经营养因子-3的表达上调80.54%(P<0.01),凋亡细胞百分比也显著降低(P<0.05)。结论:低浓度牛蒡苷元对H89诱导的SH-SY5Y细胞损伤具有显著保护作用。
Objective：To investigate the protective effect of aretigenin against H89-induced human neuroblastoma SH-SY5Y cell injury. Mcthods： SH- SY5Y cells were treated with PKA inhibitor H89 to establish the cell injury model. SH-SY5Y cells were divided into normal group （normal cells）, model group （H89-treated cells）, H89＋Arc group （H89- and arctigenin-treated cells） and H89＋ Sal B group （positive control group, H89- and salvianolie acid B-treated cells）. The cell vitality, the expression of β-amyloid （Aβ） and neurotrophin-3 （NT-3） in cells and cell apoptosis rate were determined respectively by MTT assay, immunofluorescence cytochemistry staining technique and Hoeehst33258 staining method. Results： The H89-induced cell injury model was successfully established. Aretigenin exerted a maximal protective effeet on the cell injury model at low concentration （0.5 μ mol.L-1）. Compared with model group, the cell vitality was significantly increased （P〈0.01）, the expression of Aβ in cells was down-regulated by 5.17% （P〈0.05）, the expression of NT-3 in cells was up-regulated by 80,54% （P〈0.01） and the percentage of apoptotic cells was also significantly decreased （P〈0.05） in H89＋ Arc （05 μmol.L-1） group, Conclusion： Arctigenin has a significant protective effect against H89-induced SH-SY5Y cell injury at low concentration.
出处
《药学进展》
CAS
2014年第3期215-219,共5页
Progress in Pharmaceutical Sciences
基金
十一五国家科技重大专项"重大新药创制"项目(No.2009ZX09103-423)
