摘要
以猪胰脏为原料,采用异丙醇沉淀、硫酸铵盐溶盐析、透析和反渗透系列方法对淀粉酶提取纯化,并对其性质进行了研究。结果表明,制备的反渗透酶比活力达856U/mg,约为粗酶(146U/mg)的6倍,可替代市购粗淀粉酶用于实验,其最适温度和pH分别为37℃和6.9。
The amylopin was extracted from porcine pancreas, partially purified and characterized by using a combination of precipitation with isopropanol, solving- precipitating with ammonium sulfate, dialysis and reverse osmosis.The results showed that the specific activity of amylopin from porcine pancreas was 856U/mg protein, almost 6 times as that of crude enzyme（ 146U/mg）.The amylopin can be a substitution of commercially available enzyme and used in our experiments.The optimum temperature and pH value were 37℃ and 6.9 respectively.
出处
《食品工业科技》
CAS
CSCD
北大核心
2011年第1期169-172,共4页
Science and Technology of Food Industry
关键词
猪胰淀粉酶
盐溶盐析
透析
反渗透
比活力
amylopin salt solvent and salting- out dialysis
reverse osmosis specific activity
