摘要
目的建立高效毛细管电泳法（HPCE）同时测定不同批次板蓝根药材中表告依春、尿嘧啶、胸腺嘧啶、腺苷、次黄嘌呤、鸟苷、尿苷7种核苷类成分含量的方法。方法采用未涂渍标准熔融石英毛细管（75μm×64．5cm，有效长度56cm）为分离通道，60mmol·L-1硼砂-15mmol·L-1β环糊精（pH9．6）为运行缓冲液，分离电压为20kV，检测波长为254nm，毛细管温度为25℃，压力进样为5kPa×10s。结果7种核苷类成分的响应峰面积与其相应质量浓度的线性关系良好（r〉0．9994）；加样回收率为97．15％～100．40％。实验表明，不同批次板蓝根药材中7种核苷类成分的含量有所差异。结论该方法简便、可靠，重复性较好，可用于板蓝根质量的评价和控制。
OBJECTIVE To establish a high performance capillary electrophoresis （HPCE）method for simuhaneous determination of seven nucleosides, including epigoitrin, uracil, thymine, adenosine, hypoxanthine, guanosine and uridine in Isatidis Radix. METHODS The chromatographic separation was performed on an uncoated fused silica capillary （75 μm × 64. 5 cm, 56 cm of effective length）with separation voltage of 20 kV. 60 mmol . L- 1 Na2 B4 07-15 nmlol. L-1 13-CD was selected as the running buffer solution （ pH 9. 6）. The de- tection wavelength was set at 254 nm. The sample was injected at 5 kPa × 5 s and column temperature was maintained at 25℃. RE- SULTS The calibration curves of the seven nucleosides showed good linearity （ r 〉 0. 999 4）. The average recoveries of the method were between 97. 15% - 100. 40%. The contents of the seven nucleosides in Isatidis Radix samples of different batches were different. CON- CLUSION The established method is simple, accurate and c.an he used for the quality control of Isatidis Radix.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2014年第7期604-608,共5页
Chinese Pharmaceutical Journal
关键词
高效毛细管电泳
板蓝根
核苷
同时测定
HPCE
Isatidis Radix
nucleoside
simultaneous determination
