摘要
目的:建立简单、快速测定冬虫夏草和人工蛹虫草子实体中虫草素、腺嘌呤、鸟嘌呤、尿嘧啶、次黄嘌呤、腺苷、鸟苷、尿苷、次黄苷9个核苷及碱基类成分的高效毛细管区带电泳法,比较天然冬虫夏草与人工蛹虫草子实体中核苷及碱基类化合物的含量。方法:采用未涂层熔硅弹性石英毛细管(75μm×60.5 cm,有效长度50 cm),以pH=9.0的0.05 mol.L-1硼砂-硼酸(8∶2,含硼酸根为0.2 mol.L-1)作为缓冲体系,分离电压20 kV,温度20℃,检测波长258 nm,进样量为3.445 kPa,进样5 s。结果:在优化的实验条件下,冬虫夏草及人工蛹虫草子实体中上述9个核苷及碱基类成分在15 min内达到基线分离;核苷及碱基类成分的浓度在1.0～205.5μg.mL-1范围内与峰面积呈现良好的线性关系,相关系数为0.9995～0.9999,样品回收率为95.3%～106.5%。结论:人工蛹虫草子实体与天然虫草有相似的核苷及碱基成分,但在含量上有较大差异。新建的方法操作简便,实验消耗低,高效而快速,可以成为冬虫夏草及代用产品中核苷及碱基类成分检测的一种简便、价廉、有效的分析方法。
Objective：To develop a simple and rapid high performance capillary zone electrophoresis（HPCZE） method for determination of 9 nucleosides and nucleobases（cordycepin,adenine,guanine,uracil,hypoxanthine,adenosine,guanosine,uridine,inosine） in natural Cordyceps sinensis and cultured Cordyceps militaris and then compared the contents of nucleosides and nucleobases from them.Methods： The contents of nucleosides and nucleobases were determined using HPCE.P/ACETM system MDQ（American Beckman Co.） equipped with PDA detector and a fused-silica capillary（75 μm×60.5 cm,effective length：50 cm） were adopted.The running buffer solution was 0.2 mol·L-1 sodium borate buffer（pH=9.0）;The running voltage was 20 kV and the column temperature was set at 20 ℃ during separation;The wavelength of detector was 258 nm and the sampling condition was 3.445 kPa,5.0 s.Results：Under the optimized conditions,the nine nucleosides and nucleobases reach the baseline separation in 15 minutes and the nucleosides and nucleobases contents demonstrated good linear relationship in the range of 1.0-205.5 μg·mL-1（r≥0.9995）.Conclusions：There are similar nucleosides and nucleobases from natural Cordyceps sinensis and cultured C. militaris,but the contents of the nucleosides and nucleobases showed relatively large difference.The results show that this method can be utilized for the quantitative analysis of nucleosides and nucleobases in natural and cultured Cordyceps militaris and its substitutes,and it is helpful to control their quality.It has the advantages of accuracy fastness,convenience and low sample consumption.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第1期24-29,共6页
Chinese Journal of Pharmaceutical Analysis
基金
“973”项目(2006CB04701)
