摘要
目的测定不同产地人工蛹虫草子实体与天然冬虫夏草中核苷类成分的含量,为人工蛹虫草的质量评价提供依据。方法以尿苷、腺苷、虫草素为主要评价指标,采用高效液相色谱法测定9个厂家11批人工蛹虫草样品和4种来源天然冬虫夏草中核苷类成分的含量。结果不同产地人工蛹虫草的核苷含量为0.265%～0.870%,冬虫夏草核苷含量为0.168%～0.250%,其中虫草素含量变化较大,而天然冬虫夏草中几乎不含或含有少量虫草素。结论产地不同,人工蛹虫草中核苷类成分含量不同,但明显高于天然冬虫夏草中核苷类成分含量,其中腺苷、尿苷含量较为稳定,可为人工蛹虫草的合理开发利用和质量评价提供依据。
Objective To detect quality the nucleosides contained in cultured Cordyceps militaris mycelia from different habitats and natural Cordyceps sinensis for qulity evaluation. Methods The contents of nucleosides in 11 batches of cultured Cordyceps militaris from 9 different places and 4 types of natural Cordyceps sinensis were determined by using a HPLC method with the evaluating markers of uridnine, adenosine and cordycepin. Results The contents of nucleosides in cultured Cordyceps militaris mycelia were varied from 0. 27% to 0. 87% and those in Cordyceps sinensis were 0. 17% to 0. 25%. Cordycepin levels differed more obviously, less or no cordycepin was detected in natural Cordyceps sinensis. Conclusion Different contents of nucleosides are presented in cultured Cordyceps militaris mycelia from different habitats, while much higher than those in natural Cordyceps sinensis. Among which, the contents of adenosine and uridnine are constant, and could be used as valuable bases for development and quality evaluation of artificial Cordyceps militaris mycelia.
出处
《医药导报》
CAS
2009年第10期1354-1356,共3页
Herald of Medicine
关键词
蛹虫草
人工
冬虫夏草
核苷
色谱法
高效液相
Cordyceps militaris mycelia ,cultured
Natural Cordyceps sinensis
Nucleosides
HPLC
