摘要
本试验将卡介菌多糖核酸应用于新城疫疫苗免疫雏鸡,观察其抗氧化和对鸡体免疫功能的影响。将200只1日龄AA肉鸡随机分为5组,每组40只,分别为卡介菌多糖核酸高(0.12mg/只)、中(0.06mg/只)、低(0.03mg/只)剂量组,黄芪多糖组(阳性对照,0.06mg/只)和空白对照组。每组鸡7日龄时免疫新城疫疫苗并同时按分组要求注射药物,连续7d,空白对照组以生理盐水代替。分别于用药后5、10、15和20d分批剖杀鸡,采取胸腺、脾脏、法氏囊和颈静脉血,测定免疫器官系数,鸡新城疫病毒(NDV)抗体滴度,血浆IgG、IgM、超氧化物歧化酶(SOD)、脂质过氧化物(LPO)和谷胱甘肽过氧化物酶(GSH-Px)的含量。结果表明,卡介菌多糖核酸能显著提高鸡血清中SOD和GSH-Px含量,降低LPO含量(P<0.05),具有良好的抗氧化功能;能显著提高受试鸡的免疫器官系数、NDV抗体滴度、血清中IgG和IgM含量(P<0.05),具有良好的免疫佐剂效应。
Polysaccharide nucleic acid fraction of Bacillus Calmette Guerin （BCG-PSN） was applied on chicken immuned by Newcastle disease （ND） vaccine to observe the influence on antioxidation and immune activities. Two hundreds 1-day-old AA chicken were randomly divided into 5 groups,BCG-PSN high dose （0.12 mg per chick）, medium dose （0.06 mg per chick）, low dose （0. 03 mg per chick） groups, Astragalus polysaccharide group （APS, positive group, 0.06 mg per chick） and control group with 40 chicken each group. All chicken were injected with drugs for 7 days according to the group and immuned by ND vaccine in 7 days old, while the control group was injected with physiological saline instead. Chicken were killed after 5,10,15, 20 days injection, and thymus, spleen, bursal and jugular vein blood were collected, while immune organ index, NDV antibody titer, SOD, LPO, GSH-Px, IgG and IgM were tested. The results showed that BCG-PSN could not only significantly increase the activities of SOD and GSH-Px while significantly reduce the concentration of LPO （P〈0.05） which indicated a good function of antioxidation, but also significantly increase the immune organ index, NDV antibody titer, concentrations of IgG and IgM in serum （P〈0.05） which indicated an effect of immune adjuvant.
出处
《中国畜牧兽医》
CAS
北大核心
2014年第4期148-152,共5页
China Animal Husbandry & Veterinary Medicine
基金
江苏农牧科技职业学院青年科研基金项目(QN201105)
