摘要
为探讨卡介菌多糖核酸(BCG-PSN)的抗病毒效果,对离体培养的鸡胚成纤维(CEF)细胞进行水疱口炎病毒(VSV)感染,用四甲基偶氮唑盐(MTT)比色法检测BCG-PSN对CEF细胞活性的影响;用细胞病变法(CPE)检测BCG-PSN对VSV的抑制作用,并在安全浓度范围内筛选BCG-PSN抑制VSV的最佳作用时间和作用剂量。试验结果表明:BCG-PSN能显著抑制VSV在CEF细胞上的生长,但抑制作用并非随着其剂量的增加和作用时间的延长而增强,质量浓度为144.0μg/mL的BCG-PSN在CEF细胞上作用24 h后,产生的抗病毒活性最强;当BCG-PSN质量浓度大于800μg/mL时则对CEF细胞具有明显的毒性作用。BCG-PSN可诱导CEF细胞产生IFN-α和IFN-β,研究结果显示:BCG-PSN的抗病毒作用与其诱导的干扰素有关。
To investigate the anti-viral activity of Bacillus Calmette Guerin（BCG-PSN）,vesicular stomatits virus（VSV）,MTT method and CPE method were used to observe the cell viability and to detect the protection of CEF cells from VSV infection in vitro.The optimal incubation time and concentration were also determined according to MTT method.The result showed that BCG-PSN had toxicity to CEF cells after treated with more than 800 μg/mL BCG-PSN.BCG-PSN can inhibit VSV growth in CEF cells,however,the anti-viral activity was not changed with concentration induction of BCG-PSN and incubation time.The anti-VSV activity was maximal for 144.0 μg/mL of BCG-PSN treatment at 24 h.The result also showed that the anti-viral activities induced by BCG-PSN in CEF cells were related to the levels of IFN-α and IFN-β.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2011年第3期133-139,共7页
Journal of China Agricultural University
基金
国家"863"计划项目资助(2008AA10Z328)
教育部博士点基金项目(博导类)(20100097110007)
