摘要
实验分别采用CTAB法、碱法、改良型碱法、改良型SDS法等提取金黄色葡萄球菌基因组DNA，比较其提取质量，对金黄色葡萄球菌总DNA提取方法进行优化，以便高效、节约的应用于聚合酶链式反应（PCR）等分子生物学研究。结果表明：改良型SDS法稳定性好，能够有效破壁，DNA的质量高，A260/A280为1.76，介于1.75~1.85之间。提取所需时间较少，仅需40 min，有利于金黄色葡萄球菌总DNA高效、快捷的提取。
In this research, traditional methods, including CTAB method, alkaline method, modified alkaline method,and modified SDS method were applied to extract genomic DNA from Staphylococcus aures, also, the yield and quality of DNA were compared in order to establish an effective and economical method for total DNA extraction of Staphylococcus aure. Results showed Modified SDS method is an effective and economical method for DNA extraction of Staphylococcus aure, and can prompt cell lysis, reducing the time needed to only 40 min. The yields of DNA extraction is improved, and A260/A280 was 1.76, which is between 1.75-1.85.
出处
《食品研究与开发》
CAS
北大核心
2014年第3期8-10,共3页
Food Research and Development
基金
陕西省自然科学基金(No.2012JQ2011)
陕西省教育厅自然科学基金(No.12JK0712
12JK0623)
西安市未央区项目(No.201112
201209)
