摘要
利用细胞工程技术 ,首先在 1.5% Na Cl下进行筛选 ,然后在 1.0 % Na Cl下长期继代及在无盐下和 1.5% Na Cl下反复的多步选择方法。经过 7个月的连续筛选 ,分离得到耐盐性稳定的番茄耐盐变异细胞系。耐盐系在不同 Na Cl浓度下的生长情况表明 ,该耐盐系的耐盐性有了较大提高 ,接近于 1.0 % Na Cl。耐盐系和对照中可溶性糖和游离脯氨酸含量有明显差异 ,这与耐盐系具有较高耐盐性相对应。
From the cell line derived from the cotyledon and hypocotyl of seedlings of tomato( Lycopersicum esculentum Mill), a stable NaCl tolerant variant was successfully isolated by the combination use of 1.5% NaCl one step and 1.0% NaCl multi step screening last for 7 months.The FW increment of variant and wild cell in different NaCl concentrations indicated that the salt tolerance of variant is approaching 1.0% NaCl, while the wild cell died almost completely. The physiological analysis of variant and wildcell showed notable differences. Under the stress of different NaCl concentrations, the former's soluble sugar and free proline content were much higher than the latter's, this helped the variant adjusted itself to the NaCl stress better. However, the inconsistency of the FW increment and the accumulation of free proline content under higher NaCl concentrations, showed that the active function of the accumulation of free proline under salt stress is limited in a certain NaCl concentrations. When overstress happened, it might only act as a physiological index of salt injury.
出处
《青岛海洋大学学报（自然科学版）》
CSCD
北大核心
2001年第1期75-80,共6页
Journal of Ocean University of Qingdao
基金
山东省科委项目!(鲁科字 93- 2 4)资助
