期刊文献+

电晕电场辅助5-Fu作用HeLa细胞的实验研究 被引量:1

Experimental studies on HeLa by negative prick electrostatic field with 5-Fu
下载PDF
导出
摘要 探讨了电晕负静电场联合化疗药物5-氟尿嘧啶(5-Fu)对HeLa细胞生长、增殖及死亡的影响规律.以不同强度的电晕负静电场分别作用于正常体外培养的HeLa细胞和培养液中加有一定浓度5-Fu的体外培养的HeLa细胞,采用MTT法检测HeLa细胞活性,用流式细胞仪(FCM)检测细胞死亡率.发现单纯电晕电场既能促进HeLa细胞的生长(较低电压U≤2kV的条件下),激发了细胞活性,也能够抑制细胞的生长增殖(U≥6kV的条件下),提高细胞的死亡率.而任何强度的电晕电场与5-Fu同时作用,则对HeLa细胞只具有极大的抑制作用,而且对HeLa细胞具有更大的杀伤力,这种杀伤力随电晕电场强度的增加而增加.结果表明:不同强度单纯电晕负静电场对HeLa细胞生长产生不同的促进或抑制效应;电晕负静电场联合化疗药物5-Fu则增强了对HeLa细胞的毒性作用. To determine the affect rule of negative prick electrostatic field (NPESF) combined ehemotherapeutics 5-Fu to growth, proliferation and death to HeLa cell. HeLa cells were treated by different intensities of NPESF and certain density 5-Fu. MTT method test cell activity. Flow cytometry were adopted to detect the mortality rate of cells. Simple negative prick electrostatic field can promote the growth and stimulate the activity of HeLa cells(low voltage under 2KV conditions), which can inhibit the cell growth and proliferation (high voltage greater than 6 kV conditions) and increase cell death. Any intensity of negative prick electrostatic field and 5-Fu affected at the same time has more lethality to the HeLa cells which greatly improve the cell death and showed the increase trend with the increasing of electric field strength. Different intensities of NPESF on the growth of HeLa cells produce different promoting or inhibiting effect ,but NPESF can increase the cytotoxicity of 5-Fu for Hela cells.
出处 《东北师大学报(自然科学版)》 CAS CSCD 北大核心 2014年第1期88-91,共4页 Journal of Northeast Normal University(Natural Science Edition)
基金 吉林省科技发展计划项目(20080734)
关键词 电晕负静电场 HELA细胞 5-FU MTT 流式细胞术 negative prick electrostatic field HeLa cell 5-Fu flow cytometry
  • 相关文献

参考文献9

二级参考文献44

共引文献13

同被引文献14

  • 1YAGHI O M, LI G, LI H. [J]. Nature, 1995, 378: 703-705. 被引量:1
  • 2YAGHI O M, KEEFFE M O, OCKWIG N W, et al. [J]. Nature, 2003, 423:705-714. 被引量:1
  • 3KITAGAWA S,KITAURA R, NORO S, [J]. Angew Chem,2004,43:2334-2375. 被引量:1
  • 4F/~REY G, MELLOT-DRAZNIEKS C, SERRE C, et al. [J]. Science, 2005, 309: 2040-2042. 被引量:1
  • 5LEE J, FARHA O K, ROBERTS J, et al .[J].Chem Soc Rev,2009,38; 1450-1459. 被引量:1
  • 6MURRAY L J, DINCA M, LONG J.R.[J].Chem Soc Rev,2009,38:1294-1314. 被引量:1
  • 7LI J R, KUPPLER R J, ZHOU H C. [J]. Chem Soc Rev, 2009, 38:1477-1504. 被引量:1
  • 8REITER W J, POTT K M, TAYLOR K M L, et al.[J].J Am Chem Soc,2008,130:11584-11585. 被引量:1
  • 9TAYLOR-PASHOW K M L, DELLA ROCCA J, XIE Z, et al. [J]. J Am Chem Soc, 2009, 131:14261-14263. 被引量:1
  • 10SMALDONERA,FORGANRS,FURUKAWAH,eta1.[J].AngewChemIntEd,2010,49:8630-863. 被引量:1

引证文献1

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部