摘要
目的研究自噬相关基因Beclin1与PTEN的表达与子宫腺肌病的关系,旨在为子宫腺肌病的发生发展以及治疗提供理论依据。方法用免疫组化法检测实验组子宫腺肌病在位内膜30例(增生期16例,分泌期14例),异位内膜30例(增生期17例,分泌期13例)和对照组正常子宫内膜32例(增生期22例,分泌期10例)中自噬相关基因Beclin1与PTEN在各组内膜中的表达情况。结果 (1)Beclin1在在位内膜中的阳性表达率明显低于正常子宫内膜,比较差异有统计学意义(P<0.0167),Beclin1在异位内膜中的表达率显著低于在位内膜和正常子宫内膜,两组间比较差异有统计学意义(P<0.0167);比较病例组增殖期与分泌期Beclin1的表达情况,差异无统计学意义(P>0.05)。(2)PTEN在正常子宫内膜中高表达,在子宫腺肌病异位内膜中低表达(P<0.0167),而在腺肌病的在位内膜中的表达强度介于上述两者之间(P<0.0167)。PTEN在在位内膜及异位内膜中增生期的表达均低于分泌期,差异有统计学意义(P<0.05)。(3)Beclin1蛋白与PTEN蛋白的表达呈正相关(rs=0.633,P<0.01)。结论 (1)Beclin1和PTEN在子宫腺肌病异位内膜中的阳性率低于在正常内膜和在位内膜中的表达,说明Beclin1和PTEN低表达可能与子宫腺肌病的发生发展有关。(2)Beclin1和PTEN的表达呈正相关,提示两者在子宫腺肌病的发生发展中起到重要作用。可能由于Beclin1和PTEN蛋白的缺失,导致了子宫腺肌病的发生和发展。
Objective：To study the relationship between the expression of autophagy-related genes Beclin1 and PTEN and adenomyosis and to provide theoretical basis for the development and clinical treatment of adenomyosis. Methods The expression of autophagy-related genes Beclin1 and PTEN was detected with immunohistochemistry in 30 cases with eutopic endometrium （16 cases at hyperplasia phase , 14 cases at secretory phase）, 30 cases with ectopic endometrium （17 cases at hyperplasia phase ,13 cases at secretory phase ） and 32 cases of normal endometrium as contasat group （22 cases at hyperplasia phase, 10 cases at secretion phase）. Results （1） The positive expression rate of Beclin1 in endometrium in experimental group was apparently lower than that in contrast group and the difference was statiscally significant （P＜0 0167）. The expression rate of Beclin1 in the ectopic endometrium, was significantly lower than that in ectopic and normal endometrium, it has statistically significant difference between these three groups （P＜0 0167）; there was no statistical significance of Beclin1 in the eutopic endometrium, ectopic endometrial secretory phase and proliferative phase expression （P＞0 05）.（2） The expression of PTEN in ectopic endometrium was the lowest in these three groups. The PTEN expression in normal endometrium was the highest in them and in ectopic endometrium of adenomyosis it was the lowest （P＜0.0167）. The PTEN expression of eutopic endometrium of adenomyosis was between that of normal endometrium group and that of ectopic endometrium of adenomyosis group （P＜0 0167）. The positive expression rate of PTEN in ectopic and ectopic endometrial hyperplasia period was below that of the secretory phase, and there was significant difference （P＜0 05）. （3） The protein expressions of Beclin1 and PTEN were positively correlated （rs＝0.633, P＜ 0 01）. Conclusion （1） In the ectopic endometrium, the negative expression of Beclin1 and PTEN is significantly lower than
出处
《泰山医学院学报》
CAS
2013年第12期901-904,共4页
Journal of Taishan Medical College
