摘要
目的:探讨PTEN蛋白在多囊卵巢综合征(PCOS)患者子宫内膜中的表达及其临床意义。方法:采用免疫组化法对36例PCOS患者(PCOS组)和28例非PCOS患者(对照组)子宫内膜组织进行PTEN蛋白水平检测。PCOS组对象同时测定血清生殖激素6项、空腹血糖和胰岛素,并行子宫内膜病理学检查,根据子宫内膜病理检查结果进一步分为PCOS子宫内膜病变组(11例)、PCOS子宫内膜正常组(25例);根据有无胰岛素抵抗分为PCOS胰岛素抵抗组(20例)和PCOS无胰岛素抵抗组(26例);根据体重指数分为PCOS超重和肥胖组(28例)及PCOS体重正常组(8例)。比较各组子宫内膜PTEN蛋白的表达水平。结果:子宫内膜中PTEN蛋白的表达水平PCOS组低于对照组(P<0.05);PCOS子宫内膜病变组低于PCOS内膜正常组(P<0.05)和对照组(P<0.01);PCOS胰岛素抵抗组与PCOS无胰岛素抵抗组比较无差异(P>0.05),PCOS胰岛素抵抗组低于对照组(P<0.01),PCOS无胰岛素抵抗组与对照组比较无差异(P>0.05);PCOS超重和肥胖组低于PCOS体重正常组(P<0.01)和对照组(P<0.01),PCOS体重正常组与对照组比较无差异(P>0.05)。PCOS子宫内膜病变组PTEN蛋白表达的缺失率高于PCOS内膜正常组和对照组(P均<0.01),PCOS子宫内膜正常组与对照组比较无差异(P>0.05)。子宫内膜中PTEN蛋白表达水平的下降或缺失与PCOS患者子宫内膜病变有关,且与胰岛素抵抗相关。结论:检测PCOS患者子宫内膜PTEN蛋白的表达水平,对了解PCOS子宫内膜病变有重要的参考价值。
Objective：To explore the expression and clinical significance of a tumor suppressor gene,PTEN,in endometrium of polycystic ovary syndrome （PCOS） patients.Methods：PTEN of endometrial specimen were detected with immuno histochemistry in 36 PCOS patients （PCOS group） and 28 women without PCOS （control group）.Six kinds of serum sex hormone,fasting plasma glucose and insulin were also detected in the PCOS group,and pathological examination was conducted as well.Women in PCOS group were redivided into endometrial lesion group and normal endometrium group according to the pathological result,insulin resistance group and no insulin resistance group,and overweight and obesity group and normal weight group according to body mass index.Results：The expression level of PTEN in endometrium of PCOS group was significantly lower than that in the control group （P ＜ 0.05）.The expression level of PTEN in endometrial lesion group was significantly lower than those in normal endometrium group （P ＜ 0.05）and the control group （P ＜ 0.001）.Between the insulin resistance group and no insulin resistance group,there was no significant difference in the expression level of PTEN.The expression level of PTEN in insulin resistance group were significantly lower than that in the control group （P ＜ 0.05）.There was no significant difference in the expression level of PTEN between no insulin resistance group and the control group.The expression level of PTEN in PCOS overweight and obesity group was significantly lower than that in normal weight group （P ＜0.01） and the control group （P ＜ 0.001）.Between the normal weight group and the control group,there was no significant difference in the expression level of PTEN.The negative rate of PTEN expression in endometrial lesion group was sigrificantly lower than that in normal endometrial group and the control group （P all ＜ 0.01）.There was no significant difference in the negative rate of PTEN expression between normal endometrial group of PCOS
出处
《中国计划生育学杂志》
2013年第9期607-610,共4页
Chinese Journal of Family Planning
基金
天津市卫生局科技基金项目(06KZ52)
