摘要
目的了解食用煤氟污染的粮食对雌鼠阴道开张日龄、动情周期及生殖内分泌功能的影响。方法选用90只断乳2周、体质量60～80g的SD雌性大鼠，将大鼠按体质量采用随机数字表法分为对照组、中氟组、高氟组，每组30只。各染氟组通过喂饲含不同比例的燃煤型氟中毒病区煤烘烤玉米（含氟量分别为47．8、96．0mg／kg）复制燃煤型氟中毒模型，对照组的玉米饲料来自于非病区（含氟量为5．2mg／kg）。分别于染氟60、120、180d后，分3批动情期处死大鼠，每组每批10只。喂养初期，每天早上8：00观察并记录雌鼠阴道开张情况。处死前连续10d，雌鼠行阴道脱落细胞涂片，HE染色后判别动情周期。于动情期股动脉取血检测雌激素（E2）水平。结果高氟组雌鼠阴道开张日龄[（49．0±4．5）d]长于对照组、中氟组[（45．4±2．3）、（46．7±1．9）d，P均〈0．05]。染氟120d，高氟组的雌鼠动情周期[（5．8±0．8）d]长于对照组、中氟组[（4．4±0．9）、（4．9±0．7）d，P均〈0．05]。染氟180d，对照组、中氟组、高氟组的雌鼠动情周期[（4．3±0．7）、（5．4±1．1）、（6．7±0．9）d]依次延长（P均〈0．05）。在高氟组，染氟180d雌鼠的动情周期[（6．7±0．9）d]高于染氟60、120d[（5．2±1．1）、（5．8±0．8）d，P均〈0．05]。染氟60d时，中、高氟组雌鼠E2含量[（438．75±71．80）、（456．25±57．80）ng／L]均高于对照组[（301．25±94．17）ng／L，P均〈0．05]，随染氟剂量增加而逐渐升高；仅高氟组在180dE2水平[（275．00±127．83）ng／L]低于60、120d[（456．25±57．80）、（392．50±102．50）ng／L，P均〈0．05]。结论燃煤型氟中毒可导致雌鼠开张日龄和动情周期延长及生殖内分泌水平改变，且染氟剂量越多、染毒时间越长，影响越重。
Objective To observe the impact of coal-burning-borne fluorosis on the opening day of vagina, and estrogen cycle and endocrinology of female rat. Methods Ninety weaned two weeks and healthy female SD rats, weighing 60 - 80 g were selected, and divided into three groups by body weight using random number table： control group, moderate fluoride exposed group, and higher fluoride exposed group, thirty rates in each group. Rats in fluoride exposed groups were fed with baked corn collected from coal-burning-borne fluorosis areas containing different levels of fluorine（fluorine contents were 47.8 and 96.0 mg/kg, respectively） to establish coal-burning- borne fluorosis models. The corn fed to control group rats were collected from non-endemic areas （fluoride content was 5.2 mg/kg）. After exposed to fluoride for 60, 120 and 180 days, the rats were put to death during content in three batches （10 rats in every batche）. The situation of female vaginal opening was observed and recorded ever/ morning at 8：00 at the beginning of the experiment. Ten female rats in each group were randomly selected to observe vaginal exfoliated cells for 10 days continuously every morning, and HE staining was selected to discriminate estrogen cycle. Femoral artery blood was collected to observe the impact of estradiol（E2） in cstrus. Results The vaginal opening day age of the longer fluoride exposed group [ （49.0 ± 4.5）d] was ] than that of the control group[ （45.4± 2,3）d] and the moderate fluoride exposed grnup[（46.7 ±1.9）d, all P 〈 0.05]. Estrogen cycle of the longer fluoride exposed group[ （5.8 ± 0.8）d] after fluoride exposure for 120 d was higher daan that of the control group [（4.4 ± 0.9）d] and the moderate fluoride exposed group [ （4.9 ± 0.7）d, all P 〈 0.05]. Estrogen cycle increased with increasing dosage of fluoride after fluoride exposure for 180 d in three groups[control group： （4.3 ± 0.7）d, moderate fluoride exposed group： （5.4 ± 1.1）d, higher fluoride exposed group�
出处
《中华地方病学杂志》
CAS
CSCD
北大核心
2014年第2期138-140,共3页
Chinese Journal of Endemiology
基金
贵州省科技厅项目[黔科合LG（2011036）]
贵州省科技厅项目[黔科合J字（20112277）]
教育部财政部（教高函200918）
贵州省贵阳医学院医学检验学院科研项目
关键词
氟中毒
牙
煤
雌二醇
鼠
Fluorosis, dental
Coal
Estradiol
Rats
