摘要
目的:探讨模拟失重对雌性大鼠生殖功能的影响及其发生机制,为太空探索者的健康保护提供可借鉴的科学线索。方法:利用尾部悬吊法建立模拟失重状态大鼠模型;选取Wistar雌性大鼠72只,随机均分为6组,3个实验组分别为:模拟失重7d组、模拟失重14d组和模拟失重21d组;3个对照组是与模拟失重组相对应的自由活动组。应用酶联免疫吸附法(ELISA)检测血清中雌二醇(E_2)、卵泡刺激素(FSH)和黄体生成素(LH)水平;HE染色观察卵巢组织结构,特异性脂褐素染色观察卵巢组织衰老状况;逆转录定量PCR(qRT-PCR)技术检测细胞衰老相关分子端粒酶逆转录酶(TERT)、p53、p16、p21和P27的mRNA表达水平;免疫组织化学方法检测P53、P16、P21和P27的蛋白表达;随机取健康雄性大鼠分别与各组雌性大鼠合笼,比较生育仔鼠数量和存活率。结果:与各对照组相比,模拟失重7d组、14d组和21d组大鼠血清中E_2下降,FSH和LH上升(P<0.05);大鼠卵巢组织卵泡数量明显减少,卵巢组织发生细胞衰老现象,细胞衰老相关分子TERT mRNA表达被抑制(P<0.05),衰老相关的细胞周期调控分子p53、p16、p21和p27在mRNA和蛋白水平表达均升高(P<0.05)。各模拟失重组雌性大鼠生育的仔鼠数量较相应的对照组明显减少(P<0.05),仔鼠存活率显著下降(P<0.01)。结论:尾悬吊模拟失重导致雌性大鼠生殖功能下降,该现象与模拟失重诱导卵巢组织细胞衰老和下丘脑-垂体-卵巢轴内分泌功能紊乱相关。
AIM：To investigate the effects of weightlessness simulated by tail-ventrofixation on reproductive functions in female rats and the underlying mechanism,and to provide some clues to health protection for the space explorers. METHODS：To mimic the weightless state,the rat model of tail-ventrofixation was set up.Seventy-two female Wistar rats were randomly divided into 6 groups：7-day,14-day and 21-day tail-ventrofixation groups and the corresponding 3 control groups without tail-ventrofixation.The levels of estradiol（E_2）,follicle stimulating hormone（FSH） and luteinizing hormone（LH）in the serum were detected by ELISA.The morphological characters of the ovary were examined under microscope with HE staining.The character of senescence was checked by senescence-specific lipofuscin staining in situ.The RNA of the ovary was extracted to detect the mRNA expression levels of telomerase reverse transcriptase （TERT）,p53,p27,p21 and P16 by qRT-PCR.The protein levels except TERT were also detected by immunohistochemistry. The male Wistar rats cohabited with the female ones to test the quantity and the survival rate of their offspring.RESULTS： Compared with the corresponding control groups,the serum level of E2 decreased in tail-ventrofixation groups （P〈0.05）.However,the levels of FSH and LH increased（P〈0.05）.At the same time,the number of ovarian follicle in tail-ventrofixation groups reduced compared with the corresponding control groups,and the ovary cells showed the characteristics of senescence.The expression of TERT was inhibited in tail-ventrofixation groups compared with the corresponding control groups not only in the level of mRNA but also of the proteins（P〈0.05）.Meanwhile,the expression of p53,p27 and pl6 increased（P〈0.05）.Both the number and the survival rate of offspring rats decreased in tail-ventro- fixation groups as compared with the corresponding control groups（P〈0.01）.CONCLUSION：The state of simulated weightlessness results in decreased reproductive funct
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2011年第4期769-774,共6页
Chinese Journal of Pathophysiology
关键词
尾悬吊
失重
卵巢
生殖
Tail-ventrofixation
Weightlessness
Ovary
Reproduction
