摘要
为了研究鸡蛋清中卵转铁蛋白的分离提取工艺，采用硫酸铵沉淀和离子交换层析分离卵转铁蛋白，用聚丙烯酰胺凝胶电泳（SDS—PAGE）检测其纯度。鸡蛋清原液用Tris—HCl缓冲液稀释5倍，4℃下静置6h以上，采用50％～80％硫酸铵分级沉淀和Q—Sepharose FF离子交换层析分离卵转铁蛋白。结果显示，分离提取的卵转铁蛋白纯度达到96％，回收率为88．63％，且其具有生物活性，对大肠杆菌的抑菌率为48．84％。表明采用硫酸铵分级沉淀和Q—SepharoseFF离子交换层析法分离鸡蛋清中卵转铁蛋白可行。
To study separation processing of ovotransferrin from egg white proteins, ovotransferrin was separated by ammonium sulfates and anion-exchange chromatography. Purity of ovotransferrin was assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDSPAGE）. Diluted egg white with five times volume of Tris HC1 buffer solution, deposited for at least 6 hours at 4 ℃ ,was treated by fractional precipitation with ammonium sulfate（from 50% to 80%） and Q-Sepharose FF anion-exchange chromatography. It showed that the purity of ovotransferrin was 96% by SDS-PAGE detection,the recovery was 88.63%,and bacteriostasis rate against E. coli was 48.84%. Ammonium sulfate precipitation and Q-Sepharose FF ion exchange chromatography was feasible to separate ovotransferrin from egg white.
出处
《河南农业科学》
CSCD
北大核心
2014年第3期158-160,共3页
Journal of Henan Agricultural Sciences
基金
丽水市科技局校企项目(20080410)
关键词
鸡蛋清
卵转铁蛋白
分离提取
离子交换层析
egg white
ovotransferrin
separation
anion-exchange chromatography
