摘要
在不同温度下对卵白蛋白的粒径、Zeta电位、巯基含量、内源荧光、表面疏水性等理化性质以及酶解后卵白蛋白的水解度、血管紧张素转化酶(angiotensin converting enzyme,ACE)抑制活性进行研究。结果表明:随着热处理温度的增加,卵白蛋白粒径分布发生了变化,平均粒径先增大后减小,从(622.73±4.60)nm增大到(1527.00±59.40)nm,然后减小至(261.64±2.26)nm;Zeta电位绝对值从(6.56±0.36)mV增大到(19.20±0.62)mV;表面巯基含量从(2.20±0.37)μmol/g增加到(23.76±0.71)μmol/g,总巯基含量则先减少后增大,从(9.63±0.26)μmol/g减少到(6.46±0.16)μmol/g,然后增至(29.55±1.88)μmol/g;内源荧光最大吸收峰发生1 nm的蓝移;表面疏水性值先增大后减小,从(249.52±0.49)增大至(274.32±1.19),然后减小至(135.17±14.81);水解度先增大后减小,60℃时可达到(28.62±0.42)%;ACE抑制活性有着不同程度的增大,80℃时的ACE抑制活性达到(79.17±0.54)%,比未处理溶液的ACE抑制活性高了23.01%。
The physicochemical properties of ovalbumin,particle size,Zeta potential,sulfhydryl content,intrinsic emission fluorescence spectra,surface hydrophobicity,and degree of hydrolysis of ovalbumin and angiotensin converting enzyme(ACE)inhibition activity were studied at different temperatures.The results showed that the grain size distribution of ovalbumin after heat treatment changed,and the average grain size first increased and then decreased,from(622.73±4.60)nm to(1527.00±59.40)nm,and then decreased to(261.64±2.26)nm.The absolute value of Zeta potential increased from(6.56±0.36)mV to(19.20±0.62)mV.The surface sulfhydryl content increased from(2.20±0.37)μmol/g to(23.76±0.71)μmol/g,and the total sulfhydryl content decreased from(9.63±0.26)μmol/g to(6.46±0.16)μmol/g,and then increased to(29.55±1.88)μmol/g;the maximum absorption peak of endogenous fluorescence occurred 1 nm blue shift;the surface hydrophobicity first increased and then decreased,from(249.52±0.49)to(274.32±1.19),and then decreased to(135.17±14.81);the degree of hydrolysis first increased and then decreased,reaching(28.62±0.42)% at 60℃;the inhibitory activity of ACE increased to different degrees,reaching(79.17±0.54)%at 80℃,ACE inhibition activity was 23.01%higher than that of untreated solution.
作者
张晓
杨锋
赵笑蕾
ZHANG Xiao;YANG Feng;ZHAO Xiao-lei(Guangxi University of Science and Technology,Liuzhou 545006,Guangxi,China)
出处
《食品研究与开发》
CAS
北大核心
2020年第11期34-40,共7页
Food Research and Development
基金
广西自然科学基金项目(2016GXNSFAA380025)。
关键词
热处理
卵白蛋白
理化性质
水解度
血管紧张素转化酶抑制活性
heat treatment
ovalbumin
physicochemical properties
degree of hydrolysis
ACE inhibition activity
