摘要
目的:研究人参茎叶总皂苷对幼年雄性大鼠生殖系统的影响及作用机制。方法:将40只SD幼年大鼠分为对照组及人参茎叶总皂苷低、中、高剂量给药组,灌胃两周后处死,用放射免疫法检测大鼠血清中睾酮的含量,用酶联免疫试剂盒测量大鼠血清中双氢睾酮、促黄体激素和促卵泡素的含量,称量大鼠的睾丸质量,计算体质量增加量、睾丸脏器系数和精子密度。用高效液相色谱仪对比分析高剂量组大鼠血清和对照组血清中成分的差异。将高剂量组大鼠血清和人参茎叶总皂苷溶液,按0%(对照组)、5%、10%、15%的体积分数分别加到培养的大鼠睾丸间质细胞中,用台盼蓝染色法和噻唑蓝法检测细胞存活率和活力,用放免法检测睾丸间质细胞培养液中睾酮的含量,用大鼠环磷酸腺苷酶联免疫试剂盒检测睾丸间质细胞内环磷酸腺苷的变化。用SPSS软件分析各组间差异。结果:人参茎叶总皂苷能剂量依赖性地显著降低幼年大鼠的血清睾酮水平(P<0.01)、双氢睾酮(P<0.01)水平和精子密度(P<0.01)。与对照组相比,高剂量组大鼠血清中含有5种成分是对照组中所没有的,并且人参茎叶总皂苷给药组的睾丸质量(P<0.05)和体质量增加量(P<0.01)显著降低,血清促黄体激素(P<0.01)、促卵泡素(P<0.01)和睾丸脏器系数显著上升(P<0.05)。人参茎叶总皂苷及其含药血清能抑制睾丸间质细胞内内环磷酸腺苷水平和睾酮的合成。结论:人参茎叶总皂苷可能通过抑制蛋白激酶A途径降低大鼠血清中睾酮的生成,从而抑制睾丸发育和精子生成,影响雄性幼鼠生殖系统的发育。
Objective： To study the effect of total ginsenosides from ginseng stems and leaves（TGSL） on the reproductive system of juvenile male rats and its mechanism. Methods： Forty SD rats were randomized into control group and three administration groups. The administration groups and control group were given TGSL at high, middle and low doses and water by intragastric administration, respectively. After administration for two weeks, testosterone, dihydrotestosterone（DHT）, luteinizing hormone（LH）, follicle-stimulating hormone（FSH）, testis weight, body weight, testis weight coefficient and sperm density were tested. The difference in serum components between high dose group and control group was compared by HPLC. TGSL and serum from the high-dose group were added into the culture medium of rat leydig cells, respectively, at volume fractions of 0（control group）, 5%, 10% and 15%. Leydig cell viability and vitality, the content of testosterone in cell culture medium and intracellular cAMP were measured. The content of testosterone was tested by radioimmunoassay. DHT, FSH, LH and intracellular cAMP were measured by ELISA. Cell viability and vitality of cultured rat leydig cells were tested by placenta blue staining and MTT assay. Finally, SPSS software was used to analyze the differences among different groups. Results： The contents of testosterone and DHT in serum and sperm density of three administration groups significantly reduced in a dosedependent manner by total ginsenosides（P ＆lt; 0.01）. Compared with the control group, testis weight（P ＆lt; 0.05） and the increase of rat body weight（P ＆lt; 0.01） in the administration groups significantly decreased, but the contents of LH and FSH（P ＆lt; 0.01） in serum and testis weight coefficient（P ＆lt; 0.05） in the administration groups significantly increased. Five compounds were detected in the serum from the high dose group with HPLC, but were not observed in the serum from the control group. The secretion of testosterone and
出处
《食品科学》
EI
CAS
CSCD
北大核心
2014年第3期187-192,共6页
Food Science
基金
国家自然科学基金面上项目(30973956)
关键词
人参茎叶总皂苷
睾酮
精子
促黄体激素
促卵泡素
total ginsenosides from ginseng stems and leaves
testosterone
spermatozoa
luteinizing hormone
follicle-stimulating hormone
