摘要
目的:探讨参芪花粉片抗疲劳的作用特点。方法:将70只雄性SD大鼠随机分为空白组、模型组、参芪花粉片高、中、低剂量组(2.5,1.25,0.625 g·kg-1)、硝苯地平片组(0.01 g·kg-1)、刺五加片组(0.25 g·kg-1)。连续ig给药9 d。采用负重游泳实验造大鼠力竭模型。测定体重、肝脏中三磷酸腺苷酶(ATP)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、肾脏中丙二醛(MDA)、超氧化物歧化酶(SOD)、血乳酸(LD)等指标。结果:与空白组比较,模型组ATP,CAT,GSH-Px,MDA,SOD,血LD等指标显著降低(P<0.01)。高、中剂量参芪花粉片组体重均大于模型组(P<0.01)。与模型组相比,高剂量参芪花粉片组可明显改善肝脏Na+-K+-ATP酶、Mg2+-ATP酶和Ca2+-ATP酶活性(P<0.05),显著提高肝脏GSH-Px活性(928.4±150.1)U·mL-1(P<0.01)和肾脏SOD活性(289.0±4.2)UN·mL-1(P<0.01);高、中剂量参芪花粉片组均可显著提高肝匀浆CAT活性(399.2±78.1),(372.0±139.9)U·g-1(P<0.01),显著降低肾脏MDA水平(1.93±0.33),(2.18±0.47)nmol·mg-1(P<0.01),明显降低血LD水平(7.84±1.18),(9.69±1.36)mmol·L-1(P<0.05)。结论:参芪花粉片可显著增强大鼠的抗疲劳能力,其作用可能与抗氧化和加速乳酸代谢有关。
Objective：To study of Shenqi Huafen Pian on exercise-induced fatigue in rats.Method：Seventy male SD rats were randomly divided into normal control group,model control group,Shenqi Huafen Pian high,medium,low dose groups （2.5,1.25,0.625 g ·kg-1）,Nifedipine tablets groups （0.01 g ·kg-1）,Ciwujia tablets groups （0.25 g ·kg-1）.Drugs were administered to mice for 9 days.Except control group,the other 6 groups were conducted exhaustive swimming experiment.The weight,MDA （malonaldehyde） and lactic acid （LD）content,adenosine triphosphatase （ATPase）,catalase （CAT）,Glutathione peroxidase （GSH-Px） and superoxidedismutase （SOD） activity were measured.Result：The weight in Shenqi Huafen Pian high,medium dose groups was increased （P ＜0.01）.In high dose group of Shenqi Huafen Pian,Na＋-K＋-ATPase activity,Mg2＋-ATPase and Ca2＋-ATPase activity of liver cells were obviously increased compared with model （P ＜ 0.05）,GSH-Px activity in liver was significantly higher than that in model group （P ＜ 0.01）,activities of SOD in kidney were significantly higher than that （P ＜ 0.01）.In high and middle dose group of Shenqi Huafen Pian,CAT activities in liver were significantly higher than that in model group （P ＜ 0.01）,MDA content in kidney was lower than that （P ＜0.01）,blood LD contents were obviously lower than that in model group （P ＜ 0.05）.Conclusion：Shenqi Huafen Pian showed anti-fatigue effects.The effects could be mediated by increasing the ability of antioxidation and improving lactate metabolism.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第5期177-179,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
省院合作项目(102106000022)
关键词
参芪花粉片
力竭运动
抗疲劳
Shenqi Huafen Pian
exhaustive exercises
anti-fatigue
