摘要
目的探讨1型糖尿病(type 1 diabetes mellitus,T1DM)小鼠胰岛CD3、CD57阳性细胞的表达变化及意义。方法正常雄性C57BL/6J小鼠104只,随机分为实验组、盐水对照组和正常对照组。模型诱导采用连续多次小剂量链脲佐菌素给药法(multiple low-dose of streptozotocin,MLDSTZ)。分别于注射后第3、7、10、14、21及28天测空腹血糖、取胰组织,应用免疫组织化学SABC单染法、图像分析及形态计量法进行研究。结果 1)CD3阳性细胞散在分布于胰岛内,实验3 d开始面数密度(NA)逐渐增大,以14 d组最大,之后有所减小,除28 d组外,均大于对照组,比较有显著性差异(P<0.01)。2)正常及盐水对照组CD57阳性细胞主要分布于胰岛周边,实验组CD57阳性细胞除分布于胰岛周边,还可见于胰岛中央。NA从实验3 d开始增大,以14 d组最大,之后有所减小,均高于对照组,比较有显著性差异(P<0.01)。结论实验组CD3、CD57阳性细胞数量增多,变化趋势相似,提示T1DM初期,T淋巴细胞和NK细胞可能同时浸润胰岛,协同作用胰岛发生自身免疫反应,破坏胰岛B细胞,产生T1DM;随着胰岛B细胞进行性受损,数量减少,胰岛自身免疫反应相对减弱,淋巴细胞也相应减少。
Type 1 diabetes mellitus （T1DM） results from a T-cell mediated autoimmune destruction of the pancreatic beta cells in genetically predisposed individuals, and T-cells, B-cells and NK cells play an important role on the pathogenesis of T1DM. CD3 and CD57 are the marks of T-cells and NK cells reseparately. Up to now, the situ study of T-cells and NK cells expressed in T1DM pancreatic islet is rarely. This study was designed to investigate the CD3 and CD57 positive cells population in pancreatic islet during the development of T1DM following multiple low-dose streptozotocin administration in mice. For this purpose C57BL/6J male mice were injected with streptozotocin （40 mg/kg body weight for 5 days）. Development of hyperglycemia was monitored over 28 days and a morphometric analysis of islet CD3 and CD57 positive cells was performed. Hyperglycemia was present from day 14 onwards and in parallel with hyperglycemia, insulitis developed. The image analysis of CD3 and CD 57 positive cells revealed CD3 positive cells scattered in the islet, and CD57 positive cells of the control group were around the islet, while in experiment group, there were not only distribution in the periphery but also in the middle of the islet. A raising of the numerical density on area （NA） of the two positive cells was observed after one injection of streptozotocin, with the highest value on day 14, then declined. The result prompts that T lymphocytes and NK cells may infiltrate into the islet at the same time in the early stage of T1DM, and their synergistic effect makes the islet occur autoimmune response; then with the progressive damage of islet B cells, the number of islet B cells decreases; next, the autoimmune responses of islets came to weaken; finally, lead the amount of lymphocytes reduce correspondingly.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2014年第2期105-109,共5页
Immunological Journal
基金
贵州省国际合作计划项目(黔科合外G字[2010]7017)
贵阳市科技局大学生创业基金资助项目(筑科计[2012]08)
