摘要
目的：建立用高效液相色谱-二极管阵列检测法测定新疆不同品种巴旦杏仁中槲皮素、山奈酚含量的方法。方法：色谱柱：HC-C18柱（250mm×4．6mm，5μm）；流动相：甲醇-0．4％磷酸溶液（55：45，V／V）；检测波长：360nm；柱温：30℃；流速：1．00mL／min。结果：槲皮素和山奈酚分别在1．0～300μg／mL（r=0．9993）和0．5～40μu／mL（r=0．9998）质量浓度范围内呈良好的线性关系，平均回收率分别为102．0％（相对标准偏差为2．08％，n=5）和102．8％（相对标准偏差为2．21％，n=5）。结论：该方法简便、快速、有效、准确、具有良好的重复性和回收率，可作为巴旦杏仁的质量控制和评价。
The contents of quercetin and kaempferol in almond kernels （Amygdalus communis L.） from different variet- ies were determined by a reversed phase high-performance liquid chromatography fitted with a photodiode array detector （RP-HPLC-PAD） and an Agilent HC-C18 column （5 μm, 250mm×4.6mm,5μm）. The mobile phase used was a mixture of CH3OH-0.4%H3PO4 （55：45, V/F） with a flow rate of 1.0 mL/min, and the detection wavelength was set to 360 nm. There was a good linear relationship for quercetin in the range of 1-30 μg/mL （r = 0.999 3） and kaempferol in the range of 0.5-40 μg/mL （r = 0.999 8）. The average recovery rates for quercetin and kaempferol were 102.0% （RSD = 2.08%, n = 5） and 102.8% （RSD = 2.21%, n = 5）, respectively. This analytical method was simple, rapid, sensitive and accurate, and could be used to determine quercetin and kaempferol in almond kernels with good repeatability and recovery rate.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2014年第2期182-185,共4页
Food Science
基金
中国科学院"西部之光"计划项目(RCPY201007)
