摘要
目的采用高效液相色谱-二极管阵列检测方法,建立昆仑雪菊中绿原酸、芦丁、槲皮素和木犀草素的测定方法。方法色谱柱为安捷伦HC-C18柱(250 mm×4.6 mm,5μm);流动相为甲醇-0.01%磷酸水溶液,梯度洗脱;检测波长315nm;柱温30℃;体积流量1.0 m L/min。结果昆仑雪菊中绿原酸、芦丁、槲皮素和木犀草素分别在2.0~60μg/m L(r=0.999 8)、5.0~100μg/m L(r=0.999 9)、1.0~40μg/m L(r=0.999 9)和0.5~40μg/m L(r=0.999 9)呈良好的线性关系,平均回收率分别为102.4%(RSD为1.05%)、98.6%(RSD为1.28%)、103.6%(RSD为0.95%)、101.2%(RSD为1.12%)。结论该方法操作简便、易行、稳定,具有良好的灵敏性、重现性和回收率,可用于昆仑雪菊药材的质量控制。
Objective The high performance liquid chromatography diode array detection method was used to establish the determination methods for the contents of chlorogenic acid,rutin,quercetin,and luteolin in Kunlun snow daisy(Coreopsis tinctoria,Coreopsis Tinctoriae Folium).Methods Agilent HC-C18 column(250 mm × 4.6 mm,5 μm) was used.The mobile phase was CH3OH(A)-0.01% H3PO4(B) for the gradient elution with flow rate of 1.0 m L/min,the detection wavelength was 315 nm and the column temperature was 30 ℃.Results There was a linear correlation between the concentration of chlorogenic acid 2.0—60 μg/m L(r = 0.999 8),rutin 5.0—100 μg/m L(r = 0.999 9),quercetin 1.0—40 μg/m L(r = 0.999 9),and luteolin 0.5—40 μg/m L(r = 0.999 9).The average recovery rates of chlorogenic acid,rutin,quercetin,and luteolin were 102.4%(RSD = 1.05%,n = 5),98.6%(RSD = 1.28%,n = 5),103.6%(RSD = 0.95%,n = 5),and 101.2%(RSD = 1.12%,n = 5),respectively.Conclusion The analysis method is simple,rapid,reproducible,accurate,and reliable.It could be used to identify and evaluate the quantitative determination of chlorogenic acid,rutin,quercetin,and luteolin contents in Kunlun snow daisy.
出处
《中草药》
CAS
CSCD
北大核心
2016年第9期1601-1604,共4页
Chinese Traditional and Herbal Drugs
基金
自治区高校科研计划重点项目(XJEDU2011147)
关键词
高效液相色谱-二极管阵列检测法
昆仑雪菊
绿原酸
芦丁
槲皮素
木犀草素
high performance liquid chromatography diode array detection method
Kunlun snow daisy(Coreopsis tinctoria Nutt.,Coreopsis Tinctoriae Folium)
chlorogenic acid
rutin
quercetin
luteolin
