摘要
建立了高效液相色谱法测定发酵液中色氨酸和酪氨酸含量的方法。采用Agilent C18柱（250mm＆#215;4.6mm,5μm ），以0.1mol/L醋酸钠（pH 4.0＆#177;0.05）-甲醇（90∶10）为流动相，流速为1.0mL＆#183;min-1，紫外检测器的检测波长为280nm，柱温30℃，结果显示，色氨酸的线性范围为50~500μg＆#183;mL-1，相关系数为r＝0.9985，平均回收率为99.3%（n＝4）；酪氨酸的线性范围为20~2000μg＆#183;mL-1，相关系数为r=0.9991，平均回收率为100.2％（n＝4）。该方法无需衍生，直接测定，简便，准确，用于发酵液中色氨酸和酪氨酸含量的测定，结果令人满意。
To establish HPLC method for the determination of fermentation liquid ammonia acid tryptophan and tyrosine. Agilent C18 column （250mm ＆#215;4.6mm,5μm ）was used. The mobile phase was 0.1mol/L sodium acetate （pH 4.0 ＆#177;0.05）-Methanol（90∶10）. The flow rate was 1.0mL＆#183;min-1 and detection wavelength was 280nm. Column temperature was 30℃. The results showed that tryptophan linear between 50~500μg＆#183;mL-1. The correlation coefficients was r=0.9985. The average recoveries was 99.3%（n=4）. Tyrosine was linear between 20~2000μg＆#183;mL-1. The correlation coefficients was r=0.9991. The average recoveries was 100.2%（n=4）. So drew the conclusions：Direct determination method without the need of derivative is simple,accurate, for the determination of content of fermentation liquid tryptophan and tyrosine.
出处
《安徽化工》
CAS
2014年第1期68-69,74,共3页
Anhui Chemical Industry
关键词
高效液相色谱
发酵液
色氨酸
酪氨酸
high efficiency liquid chromatography
fermentation liquid
tryptophan
tyrosine
