摘要
目的 探讨Hepcidin-25在多发性骨髓瘤（MM）患者不同疾病过程中的动态变化及其与疗效、预后的关系.方法 在临床诊疗的不同阶段分别采用实时定量聚合酶链反应（RT-PCR）、酶联免疫吸附法（ELISA）分析54例MM患者Hepcidin-25的mRNA及蛋白表达水平,研究Hepcidin-25的动态变化与患者临床表现的相关性及其对患者预后的临床预测价值.结果 MM患者Hepcidin-25 mRNA表达量明显高于健康对照组（0.58±0.19比0.08±0.03,P=0.001）,其蛋白质水平也明显高于健康对照组[（5.2±11.9）ng/ml比（22.5±7.1）ng/ml,P=0.019）].对MM患者随访发现,治疗后缓解患者（17例）的Hepcidin-25水平为（26.4±7.3） ng/ml,较其治疗前的（33.4±7.4） ng/ ml降低（t=5.312,P=0.021）.而治疗无效病情加重患者（37例）的Hepcidin-25水平为（55.9±12.7）ng/ml,较治疗前的（39.1 ±9.9）ng/ml增高（t=2.811,P=0.037）.治疗缓解组与治疗无效病情加重组患者生存率差异有统计学意义（P＜0.01）.治疗后缓解患者的血红蛋白（Hh）水平为124g/L,较治疗前的102 g/L升高（t=2.113,P=0.035）,与Hepcidin-25具有很好的相关性（r=-0.535,P=0.002）.而治疗无效病情加重患者的Hb水平为46 g/L,较治疗前的73 g/L降低（t=2.730,P=0.036）,与Hepcidin-25呈负相关（r=-0.642,P=0.001）.结论 MM患者的Hepcidin-25表达量较高,与慢性病贫血密切相关.较高水平的Hepcidin-25表达量对临床复发有一定的预测价值,与MM患者疗效、预后有密切关系.
Objective To study the dynamic changes of Hepcidin-25 in different disease process in patients with multiple myeloma （MM） and its relationship with therapeutic effect and prognosis.Methods The expression levels of Hepcidin-25 in 54 MM patients were analyzed by RT-PCR and ELISA.The correlations between dynamic changes of patients＇ Hepcidin-25 expression and clinical manifestations,clinical predictive value of the prognosis were researched.Results Expressions of Hepcidin-25 mRNA and protein in MM patients were significantly higher than those in normal control group [0.58±0.19 vs 0.08±0.027,P =0.001,（5.2±11.9） ng/ml vs （22.5±7.1） ng/ml,P =0.019].Hepcidin-25 expression of 17 patients remission after treatment was （26.4±7.3） ng/ml,it was significantly lower than that before treatment [（33.4±7.4） ng/ml,t =5.312,P =0.021].But the Hepcidin-25 level of the treatment ineffective patients （37 cases） significantly increased [（55.9±12.7） ng/ml vs （39.1±9.9） ng/ml,t =2.811,P =0.037].There existed obvious differences in survival curves of two groups （P ＜ 0.01）.Hb level of patients remission after treatment was 124 g/L,and significantly higher than that before treatment （102 g/L,t =2.113,P =0.035）.It had a negative correlation with Hepcidin-25 （r =-0.535,P =0.002）.But the Hepcidin-25 level of the treatment ineffective patients significantly increased （46 g/L vs 73 g/L,t =2.730,P =0.036）.It also had a negative correlation with Hepcidin-25 （r =-0.642,P =0.001）.Conclusions Hepcidin-25 expression levels in patients with MM are high,and are closely related to anemia of chronic disease.High expression of Hepcidin-25 has a predictive value for clinical effect and prognosis.
出处
《白血病．淋巴瘤》
CAS
2014年第1期30-32,37,共4页
Journal of Leukemia & Lymphoma
