摘要
目的:分析急性淋巴细胞白血病(ALL)患儿外周血hepcidin-25水平的变化,探讨其在ALL患儿中的作用机制。方法:选取我院收治的60例ALL患儿(实验组)和45例健康体检儿童(对照组),其中实验组根据化疗效果分为缓解组和未缓解组,采集外周血进行hepcidin-25和hepcidin-25 mRNA水平检测,比较实验组和对照组以及缓解组和未缓解组外周血hepcidin-25和hepcidin-25 mRNA的水平差异,绘制ROC曲线分析外周血hepcidin-25和hepcidin-25 mRNA对ALL的诊断价值。结果:实验组患儿外周血hepcidin-25和hepcidin-25 mRNA表达水平明显高于对照组,差异具有统计学意义(P<0.05);未缓解组患儿治疗前外周血hepcidin-25和hepcidin-25 mRNA表达水平较缓解组明显升高(P<0.01),缓解组患儿治疗后外周血hepcidin-25和hepcidin-25 mRNA表达水平显著低于治疗前(P<0.01);绘制ROC曲线分析发现:外周血hepcidin-25和hepcidin-25 mRNA水平对ALL的诊断曲线下面积(AUC)分别为0.759和0.771,诊断敏感性分别为71.5%和75.5%,诊断特异性分别为72.5%和75.3%。结论:ALL患儿外周血hepcidin-25水平的明显升高,与上调hepcidin-25基因的表达有关,可用于ALL的诊断和预后,且其水平的表达与化疗效果密切相关。
Objective:To analyze the changes of hepcidin-25 level in peripheral blood of children with acute lymphoblastic leukemia(ALL)and explore its mechanism.Methods:60 children with ALL(experimental group)and 45 healthy children(control group)admitted to our hospital were selected.The experimental group was divided into remission group and non-remission group according to the effect of chemotherapy.Peripheral blood was collected to detect the levels of hepcidin-25 and hepcidin-25 mRNA.The levels of hepcidin-25 and hepcidin-25 mRNA in peripheral blood of experimental group and control group,remission group and non-remission group were compared,and ROC was drawn to analyse the diagnostic value of hepcidin-25 and hepcidin-25 mRNA in ALL.Results:The expression levels of hepcidin-25 and hepcidin-25 mRNA in peripheral blood of children in the experimental group were significantly higher than those in the control group(P<0.05).The expression levels of hepcidin-25 and hepcidin-25 mRNA in peripheral blood of children in the non-remission group were significantly higher than those in the remission group before treatment(P<0.01).The expression levels of hepcidin-25 and hepcidin-25 in peripheral blood of children in the remission group were significantly lower than those before treatment(P<0.01).The ROC curve analysis showed that the area under the diagnostic curve(AUC)of hepcidin-25 and hepcidin-25 mRNA in peripheral blood was 0.759 and 0.771 respectively.The diagnostic sensitivity was 71.5%and 75.5%,and the diagnostic specificity was 72.5%and 75.3%,respectively.Conclusion:The level of hepcidin-25 in peripheral blood of children with ALL is significantly increased,which is related to the up-regulation of the expression of hepcidin-25 gene,which can be used in the diagnosis and prognosis of ALL,and the expression of hepcidin-25 is closely related to the effect of chemotherapy.
作者
沈静
姚虹
SHEN Jing;YAO Hong(Department of Clinical Laboratory,Capital Medical University Affiliated Beijing Friendship Hospital,Beijing 100050,China)
出处
《现代肿瘤医学》
CAS
北大核心
2021年第6期1028-1031,共4页
Journal of Modern Oncology
