摘要
目的 从分子生物学水平探讨绝经后健康妇女雌激素受体 (ER)基因PvuⅡ酶切位点多态性与骨密度的关系。方法 选择绝经后健康无亲缘关系妇女 2 37例 ,运用双能X线吸收骨密度仪及聚合酶链反应 限制性片段长度多态性 (PCR RFLP)方法分析ER基因PvuⅡ酶切位点多态性与骨密度的关系。结果 2 37例绝经后健康妇女PP、Pp及pp基因型频率分别为 19.8%、44 .3 %及 35 .9%。ANOVA分析显示 ,Troch部位骨密度与ER基因PvuⅡ酶切位点多态性显著相关 (P =0 .0 10 6 ) ;多元逐步回归分析发现 ,ER基因多态性与腰椎及Troch部位的骨密度变化相关性无统计学显著性意义 ,P值分别为 0 .0 998、0 .0 5 48。结论 中国上海地区绝经后健康妇女ER基因多态性与股骨Troch部位的骨密度显著相关 ,p等位基因具有一定的骨量保护作用。
Objective To investigate the possible relationship between bone mineral density (BMD)and polymorphism of the estrogen receptor (ER) gene in Chinese healthy postmenopausal women. Methods 237 unrelated healthy postmenopausal women were selected for bone mineral density determination with Dual energy X-ray absorptiometry (DEXA) and the polymorphism of estrogen receptor gene were detected with polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). Results It is shown that the distribution of ER genotype in the ER gene was as follows: PP 19.8%, Pp 44.3% and pp 35.9% respectively. ANOVA analysis indicated that BMD of trochanter major (Troch) was associated with polymorphism of ER gene ( P =0.010 6). Multiple stepwise regression analysis showed that the relationship between polymorphism of ER gene and BMD of Troch ( P =0.054 8) and lumbar vertebra 2～4 ( P =0.099 8)was not statistically significant. Conclusion It is suggested that there is a significant relationship between polymorphism of ER gene and Troch BMD in healthy postmenopausal women. p allele may be a protective factor for bone mass loss.
出处
《中华内科杂志》
CAS
CSCD
北大核心
2000年第11期743-745,共3页
Chinese Journal of Internal Medicine
