摘要
目的:探讨雌激素受体基因的PvuⅡ,XbaⅠ多态性与乙型肝炎肝硬化的关系,从基因水平上进一步探讨肝硬化的发病机制。方法:采用聚合酶链反应(PCR)-限制性片段长度多态性(RFLP)方法检测并比较了98例乙型肝炎肝硬化患者,72例慢性乙型肝炎患者,84例健康对照组的雌激素受体基因PvuⅡ和XbaⅠ多态性。结果:乙型肝炎肝硬化患者的雌激素受体基因的PvuⅡ多态性的Pp基因型和P等位基因频率明显高于健康对照组和慢性乙型肝炎组,pp基因型和p等位基因频率明显低于健康对照组和慢性乙型肝炎组,差异有统计学意义(P<0.05),PP+Pp基因型患肝硬化的风险是pp基因型的2.23倍(OR=2.23)。雌激素受体基因的XbaⅠ多态性分布在各组间比较,差异均无统计学意义(P>0.05)。结论:雌激素受体基因Pp基因型和P等位基因可能是肝硬化发病的遗传易感基因,pp基因型和p等位基因可能是肝硬化发病的保护基因。
Objective To investigate the relationship between estrogen receptor （ER） gene polymorphisms and HBV-induced cirrhosis. Methods XbaⅠ and PvuⅡ polymorphisms of ER gene were analyzed in 98 patients with HBV-induced cirrhosis, 72 patients with chronic hepatitis B, and 84 healthy controls by polymerase chain reaction-restriction fragment length polymorphism （PCR- RFLP） technique. Results The frequencies of Pp genotype and P allele of ER gene in patients with HBV-induced cirrhosis were higher than those in patients with chronic hepatitis B and controls, while the frequencies of pp genotype and p allele of ER gene in patients with HBV-induced cirrhosis were lower than those in patients with chronic hepatitis B and controls （ P 〈 0.05 ）. The risk of HBV-in- duced cirrhosis occurrence in carriers with Pp and pp genotype was 2.23 folds than that in carriers with pp genotype. There was no significant difference in frequencies of allele and genotype in XbaⅠ polymorphism among the HBV-induced cirrhosis group, the chronic hepatitis B group, and the control group（ P 〉 0. 05 ）. Conclusion Pp genotype and P allele might be the susceptibility gene for HBV-induced cirrhosis while pp genotype and p allele might be the protective gene.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2006年第3期379-382,共4页
Journal of Central South University ：Medical Science
关键词
雌激素受体
基因多态性
乙型肝炎
肝硬化
estrogen receptor
gene polymorphism
hepatitis B
hepatocirrhosis
