摘要
【目的】观察电针对易卒中型肾性高血压大鼠(RHRSP)大脑中动脉闭塞(MCAO)后不同时间点脑梗死灶皮层与颈髓RacGTP酶激活蛋白-1(Rac-1)mRNA表达的影响,探讨电针对急性脑梗死(ACI)远隔损害的可能机理。【方法】选用雄性SPF级SD大鼠行双肾双夹术复制RHRSP模型,再用线栓法复制MCAO模型,采用随机数字表法将模型大鼠分为脑梗死组、电针组、假针刺组、假手术组与高血压组。高血压组为单纯RHRSP;脑梗死组进行MCAO缺血再灌注处理;假手术组仅做手术创伤;电针组复合造模后选取督脉穴进行电针治疗,每天1次,共28 d;假针刺组将针灸针贴于大鼠百会和大椎穴处皮肤。治疗后第1、7、14、28天分别处死各组大鼠,分离出右侧大脑皮层和左侧脊髓,采用荧光定量PCR法检测Rac-1mRNA表达。【结果】(1)皮层Rac-1 mRNA表达:MCAO术后第7、14、28天,脑梗死组、假针刺组Rac-1 mRNA表达较假手术组显著降低(P<0.05),电针组Rac-1 mRNA表达较脑梗死组、假针刺组显著升高(P<0.05);(2)颈髓Rac-1 mRNA表达:MCAO术后第14、28天,脑梗死组、电针组、假针刺组Rac-1 mRNA表达较假手术组显著降低(P<0.05),电针组Rac-1 mRNA表达较脑梗死组、假针刺组显著升高(P<0.05)。【结论】脑梗死后皮层与颈髓Rac-1 mRNA表达降低是ACI远隔损害的重要原因,电针对高血压大鼠脑梗死中枢神经损伤的保护作用可能与其上调Rac-1 mRNA表达相关。
Objective To observe the effect of electroacupuncture （EA） on the expression of Rac-1 mRNA in cerebral cortex and Cervical pinal cord of stroke-prone renovascular hypertensive rats （RHRSP） with cerebral infarction induced by middle cerebral artery occlusion （MCAO） at different time, and to investigate the possible mechanism of EA for long-distance damage. Methods SPF SD male rats were randomly divided into cerebral infarction group, EA group, sham acupuncture group, sham operation group and hypertension group. Rats in the former three groups were induced stroke-prone renovascular hypertension by occlusion of bilateral kidney, and then induced MCAO with thread ligation method. Rats in the EA group were given EA on acupoints of Baihui and Dazhui, once per day, for 28 days, while rats in the sham acupuncture group were only given external application of acupuncture needle on the same acupoints. Stroke-prone renovascular hypertension was also induced in sham operation group and hypertension group, and sham operation group was given only neck incision. The expression of Rac-lmRNA in cerebral cortex and cervical spinal cord of all groups on the first, 7th, 14th and 28th day after MCAO was detected by fluorescence quantitative polymerase chain reaction （PCR） . Results The expression of Rac-1 mRNA in cerebral cortex of cerebral infarction group and sham acupuncture group was decreased on the 7th, 14th and 28th day after MCAO （P〈0.05 compared with that of sham operation group） . The expression of Rac-1 mRNA in cerebral cortex of EA group was increased （P〈0.05 compared with that of cerebral infarction group and sham acupuncture group） . The expression of Rac-1 mRNA in cervial spinal cord of cerebral infarction group, EA group and sham acupuncture group was decreased on the 14th and 28th day after MCAO （P〈0.05 compared with that of sham operation group）, and the expression level in EA group was higher than that in cerebral infarction group and sham acupuncture group （P〈0.05） . Conclus
出处
《广州中医药大学学报》
CAS
北大核心
2014年第1期66-71,共6页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(编号:81072947)
广东省自然科学基金项目(编号:8152800007000001)
关键词
急性脑梗死
针灸疗法
大脑皮层
脊髓
基因表达调控
疾病模型
动物
大鼠
Acute cerebral infarction/acup-moxibustion therapy
Cerebral cortex
Spinal cord
Gene expression regulation
Disease models, animal
Rats
