摘要
目的:观察针刺预处理对大鼠脑缺血再灌注损伤的保护作用。方法:采用4-血管阻断法对实验鼠分组进行全脑缺血及缺血后再灌注模型制作。神经元尼氏体亚甲兰特殊染色法观察大鼠脑缺血后海马CA1区神经元尼氏体变化;原位细胞凋亡检测法(TUNEL染色)及电镜观察脑缺血后大脑皮质、海马CA1区神经元凋亡情况。结果:针刺预处理组大脑皮质、海马CA1区可见棕色TUNEL染色阳性细胞核,较脑缺血组明显减少,尼氏体染色阳性细胞数针刺预处理组较脑缺血组明显增多。结论:针刺预处理可以减轻缺血后神经元损伤,抑制神经元凋亡。
Objective: To observe the protective effect of acupuncture preconditioning on rat's cerebral ischemia-reperfusion injury.Methods: The model with ischemia reperfusion injury was made by four vessels blocking method,changes of neuron nissl bodies in hippocampus CA_1 after ischemia reperfusion was observed by methylene blue special staining,the apoptosis of neuron in cerebral cortex and hippocampus CA_1 after ischemia were tested respectively by TUNEL and electron microscope.Results: The number of positive caryons by TUNEL staining in cerebral cortex and CA_1 in acupuncture preconditioning group was less than that of model,the number of positive nissl bodies in acupuncture preconditioning group was more than that of model.Conclusion:Acupuncture preconditioning can relieve the neuron injury after ischemia reperfusion,inhibit the neuron apoptosis.
出处
《中国中医药科技》
CAS
2006年第4期209-210,共2页
Chinese Journal of Traditional Medical Science and Technology
基金
辽宁省教育厅高等学校科学研究项目No.20040050
