摘要
目的观察银翘散精简方、玉屏风散提取物在鸡胚内抗病毒阻断和抑制的作用。方法采用鸡胚实验技术观察银翘散精简方、玉屏风散提取物对甲型流感病毒FM1株的阻断和抑制作用。结果流感病毒鸡胚半数感染量EID50为10-6;银翘散精简方、玉屏风散提取物对鸡胚最大无毒浓度分别为大于39.15、36.2g/L,利巴韦林最大无毒剂量为100g/L。银翘散精简方提取物对甲型流感病毒FM1株毒力阻断作用:半数有效量(ED50)为7.41g/L,治疗指数(TI)大于5.28,浓度为19.58g/L时鸡胚未感染率为100%,优于其他浓度(P<0.05);银翘散精简方提取物对甲型流感病毒FM1株毒力抑制作用:ED50为9.91g/L,TI大于3.95,浓度为39.15g/L时鸡胚未感染率为100%,优于其他浓度(P<0.05)。玉屏风散提取物对甲型流感病毒FM1株毒力阻断作用:ED50为9.57g/L,TI大于3.78,浓度为36.2g/L时鸡胚未感染率为100%,优于其他浓度(P<0.05);玉屏风散提取物对甲型流感病毒FM1株毒力抑制作用:ED50为10.38g/L,TI大于3.3,浓度为36.2g/L和18.1g/L时(两者毒力抑制作用均等)鸡胚未感染率为83%,优于浓度9.05g/L(P<0.05)。提取物在鸡胚死亡阻断观察上,在对毒力阻断作用时玉屏风散提取物优于银翘散精简方提取物,在对毒力抑制作用时银翘散精简方提取物优于玉屏风散提取物。各提取物对甲型流感病毒FM1株毒力阻断和抑制作用优于利巴韦林组(P<0.05)。结论银翘散精简方、玉屏风散提取物对甲型流感病毒FM1株有阻断和抑制作用。
Objective To observe the blocking and inhibiting effect of streamlined formula of Yinqiao powder （SF-YQP） and Yupingfeng powder （YPFP） extract on influenza-A FM1 strains in chicken embryo. Methods Chicken embryo experiment technology was used to observe the effectiveness of SF-YQP and YPFP extract on inhibiting influenza A virus （IV） FM1 strains. Results ED50 of IV infected chicken embryo was 10^-8. Themaximum of no toxicity concentration （TC0） of SF-YQP and YPFP extract was 39.15 g/L and 36.2 g/L,respectively. The TC0 of ribavirin was 100 g/L. SF-YQP extract had effect on preventing infection by IV FM1 strains. The ED50 was 7.41 g/L, and its therapeutic index （TI） was more than 5.28. When its concentration was 19.58 g/L, no infection rate in chicken embryo was 100%, and its effect was better than on other concentra- tion （P〈0.05）. SF-YQP extract had effect of inhibiting infection by IV FM1 strains. Its ED50 was 9.91 g/L, and its TI was more than 3.95. When its concentration was 39.15 g/L, no infection rate in chicken embryo was 100%, and its effect was bet- ter than on other concentration （P〈0.05）. YPFP extract also had effect of preventing infection by IV FM1 strains. Its ED50 was 9.57 g/L, and its TI was more than 3.78. When its concentration was 36.2 g/L, no infection rate in chicken embryo was 100%, and its effect was better than on other concentration （P〈0.05）. YPFP extract had the effect of inhibiting infection by IV FM1 strains. Its ED50 was 10. 38 g/L, and its TI was more than 3.3. When its concentration were 39.15 g/L and 18. 1 g/L, no in- fection rate on two concentrations was both 83%, and their effect was better than on the concentration of 9.05 g/L （P〈0.05）. On observing the death blocking of extract on chicken embryos, the blocking of YPFP extract was more effective than SF-YQP ex- tract, though, inhibition of SF-YQP extract was more effective than YPFP extract. Moreover,blocking and inhibition of both extracts were obviously better than ribavirin （P〈
出处
《中国中西医结合儿科学》
2013年第6期514-517,共4页
Chinese Pediatrics of Integrated Traditional and Western Medicine
基金
国家自然科学基金(81072975)
关键词
银翘散
玉屏风散
鸡胚
甲型流感病毒FM1株
动物
实验
Yinqiao powder （SF-YQP）
Yupingfeng powder （YPFP）
chicken embryo
influenza-A virus （IV） FM1 strains
animal,experiment
