摘要
目的比较补肾活血法与益气补血法对环磷酰胺(CTX)所致骨髓抑制小鼠血清白介素-6(IL-6)表达水平及骨髓基质细胞(BMSC)生长的影响。方法选用SPF级ICR小鼠80只,随机分为对照组、模型组、补肾活血组、益气补血组、补肾活血加益气补血(中药联合)组,分别给予相应药物灌胃21 d,给药第8天采用腹腔注射CTX 100 mg/(kg·d),连续3d,制成骨髓抑制小鼠模型。于造模后第3天、第7天检测血清中的IL-6,同时提取小鼠骨髓基质细胞分别培养14 d后进行观察。结果造模后第3天,模型组及各中药组小鼠血清中的IL-6含量较对照组均明显增多(P均<0.01),但3个中药组明显低于模型组,尤以中药联合组明显(P<0.05或0.01),补肾活血组次之(P<0.01)。造模后第7天,模型组与中药组IL-6下降,但模型组与补肾活血组仍显著高于对照组(P均<0.01),益气补血组和中药联合组IL-6水平与对照组比较无显著性差异。造模后第3天骨髓细胞经培养14 d后,模型组贴壁细胞数量减少,梭形成纤维细胞比例降低,细胞融合差;而各中药组骨髓基质细胞较模型组生长良好,胞体较大,突起丰富,且能形成少量集落,以中药联合组最佳。造模后第7天骨髓细胞经培养14 d后,各中药组贴壁细胞数量及成纤维细胞集落与对照组比较无明显差异。结论补肾活血法与益气补血法对骨髓抑制后小鼠骨髓基质细胞的坏死具有较好的防治作用,对血清IL-6分泌水平具有较好的调节作用,使其水平维持在适当的范围内,既有利于造血微环境的稳定,又可促进造血的恢复,且益气补血法优于补肾活血法,两法联合效果更优。
Objective It is to compare the influence of Bushen Huoxue Recipe and Yiqi Buxue Recipe on serum Interleu- kin 6 （ IL - 6） and growth condition of bone marrow stroma ceils （BMSC） in mouse with marrow-depressed induced by cyclo- phosphamide （CTX）. Methods Eighty SPF grade ICR mice were randomly divided into 5 groups： control group, model group, Bushen Huoxue group, Yiqi Buxue group, Bushen Huoxue combined with Yiqi Buxue group （combination group）. The mice were treated with corresponding drug by intragastric administration respectively for 21 days. the experimental model of bone marrow suppression was established at day 8 by peritoneal injection CTX 100 mg/（ kg · d） for 3 days continuously. The level of IL -6 was detected and BMSC were taken away for culturing at 3, 7 days after model establishment, and BMSC was observed after 14 days＇culture. Results The IL-6 levels in model group and all drugs groups were increased obviously than that in control group after duplicating the mold 3rd days （ all P 〈 0.01 ） , and the IL - 6 levels in drugs groups were lower than that in model group, especially in combination group was most obvious （P 〈 0.05 or 0.01 ） , Bushen Huuxue group came sec- ond （ P 〈 O. 01 ）. The IL - 6 levels in model group and all drugs groups were decreased at day 7 after modeling, but the levels in model group and Bushen Huoxue group were significantly higher than that in control group （ all P 〈0.01 ） , whi]e there were no significant difference between the other two groups aml control group. Fewer and lower density BMSC adherent cells of mod- el group were survived after model establishment 3 days, tile ratio of fusiformis fibroblast in BMSC was reduced, whereas the BMSCs in the drugs groups were grew well and formed colony, the cell body was big, the prominences were thick, the combi- nation group had the best growth. The BMSC of each group were observed after 14 days of culture, there were no significant differences in adherent cells number and fibroblas
出处
《现代中西医结合杂志》
CAS
2014年第3期232-234,247,共4页
Modern Journal of Integrated Traditional Chinese and Western Medicine
基金
国家自然科学基金资助项目(30979824)
关键词
益气补血法
补肾活血法
骨髓抑制
白细胞介素6
骨髓基质细胞
Bushen Huoxue Recipe
Yiqi Buxue Recipe
myelosuppression
Interleukin 6
bone marrow stromal cells
