摘要
目的研究癫痫发生不同时期生长相关蛋白-43(GAP-43)及其磷酸化形式(p-GAP-43)在海马齿状回中表达的时空分布情况。方法构建海人酸(KA)诱导的内侧颞叶癫痫(MTLE)模型小鼠,采用免疫组织化学、Western blot技术检测GAP-43与p-GAP-43在模型鼠癫痫发生不同时期(5d,2、5周)海马齿状回中的表达变化。结果 GAP-43在正常对照鼠及模型鼠的齿状回、门区和海马内分子层均有较高表达。与对照组相比,p-GAP-43在KA诱导癫痫发作后的5d,2、5周3个时间点的表达水平逐步下降。结论活性形式磷酸化的GAP-43在齿状回颗粒细胞中的表达水平随着癫痫发作时间的推移逐步降低,可能与硬化海马的轴树突异常重组过程相关。
Objective To explore the temporal and spatial distribution of growth-associated protein 43 （GAP-43） and phosphorylated growth-associated protein 43 （p-GAP-43） in the dentate gyrus of mesial tempo- ral lobe epilepsy （MTLE） mouse model. Methods MTLE mouse model was established by using the kainic acid （KA） induction. Immunohistochemistry and Western blotting were applied to detect the expressions of GAP-43 and p-GAP-43 in different stages of epileptogenesis. Results Both in the epileptic and control mice, high GAP-43 expression level was detected in the dentate gyrus, hilus, and inner molecular layer of hippocam- pus. Decreased p-GAP-43 expression was detected 5 days, 2 weeks, and 5 weeks after KA-induced sei- zures. Conclusion The decreased p-GAP-43 expression in the duration of seizure may play an important role in the synaptic reorganization of the sclerotic hippocampus.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2013年第6期589-594,共6页
Acta Academiae Medicinae Sinicae
基金
国家重点基础研究发展计划项目(973计划)(2013CB531301)
中央高校基本科研业务费专项资金(2012S05)
协和青年科研基金(2012J09)~~
关键词
内侧颞叶癫痫
生长相关蛋白-43
海人酸
海马齿状回
mesia! temporal lobe epilepsy
growth-associated protein 43
kainic acid
dentate gyrus
