摘要
背景:以Ⅰ型胶原蛋白改性多孔硫酸钙生物支架在体内的降解过程尚不明确,其降解产物对人成骨细胞的影响国内外也缺少相关研究。目的:观察人成骨细胞与硫酸钙/胶原膜复合型多孔生物支架降解产物的生物相容性。方法:将第2代人成骨细胞分别置于硫酸钙/胶原膜复合型多孔生物支架降解产物浸提液及含体积分数10%新生牛血清DMEM培养液中培养,培养第1,3,5,7天以MTT法检测两组细胞增殖曲线,联合会推荐法测定细胞碱性磷酸酶活性,考马氏亮蓝微板法测定总蛋白。结果与结论:在硫酸钙/胶原膜复合型多孔生物支架降解产物浸提液中培养人成骨细胞的增殖速度略高于在含体积分数10%新生牛血清DMEM培养液中培养的细胞,但差异无显著性意义(P>0.05)。两组碱性磷酸酶活性、总蛋白合成及碱性磷酸酶/总蛋白都随时间递增而增加,两组不同时间点上述指标差异均无显著性意义(P>0.05)。表明硫酸钙/胶原膜复合型多孔生物支架降解产物既不影响人成骨细胞的增殖生长,也不影响其正常生理功能,具有较好的生物相容性。
BACKGROUND： The degradation of a collagen I modified porous calcium sulfate scaffold in vivo is unclear, and its degradation product effects on human osteoblasts are rarely reported. OBJECTIVE： To observe the biocompatibility of human osteoblasts with degradation products of calcium sulfate/collagen membrane composite porous scaffold. METHODS： Passage 2 human osteoblasts were cultured in the extract of degradation products of calcium sulfate/collagen membrane composite porous scaffold and in Dulbecco＇s modified Eagle＇s medium containing 10% newborn calf serum. At days 1, 3, 5, 7, cell proliferative curves and total protein were determined using 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrezolium bromide method and Coomassie brilliant blue micro-plate method, respectively. And alkaline phosphatase activity was also detected. RESULTS AND CONCLUSION： The proliferation rate of human osteoblasts in the extract Qf degradation products of calcium sulfate/collagen membrane composite porous scaffold was slightly higher than that in the Dulbecco＇s modified Eagle＇s medium containing 10% newborn calf serum, but there was no significant difference （P 〉 0.05）. Alkaline phosphatase activity, total protein synthesis and alkaline phosphatase/total protein were increased with time in the two groups, but there was no significant difference at different time （P 〉 0.05）. These findings indicate that the degradation products of calcium sulfate/collagen membrane composite porous scaffold cannot influence proliferation and growth of human osteoblasts as well as their normal physiological functions, which have good biocompatibility.
出处
《中国组织工程研究》
CAS
CSCD
2013年第51期8869-8874,共6页
Chinese Journal of Tissue Engineering Research
