摘要
目的:建立人成骨细胞离体培养技术。方法:取健康儿童骨科手术时髂骨松质骨,采用蛋白酶分次消化法分离成骨细胞,采用形态学和碱性磷酸酶染色和定量测定鉴定。结果:实验中得到的人成骨细胞纯度高,性状良好。结论:蛋白酶分次消化法处理人髂骨松质骨可以得到纯度高、性状好的成骨细胞。
Objective：To study the way of culture human osteoblasts in vitro from ilium. Metbods：Osteoblasts are isolated by sequential enzyme digestion from iliae spongy bone of a child. The morphology is researched, alkaline phosphatase （AKP） is researched by eytoehemieal staining and quantity measurement. Results：The osteoblasts isolateded from human ilium were pureful and active. Conclusion：Pureful and active human osteoblasts can be obtained from ilium by sequential enzyme digestion.
出处
《中国中医骨伤科杂志》
CAS
2006年第4期8-9,共2页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
关键词
成骨细胞
培养
碱性磷酸酶
Osteoblasts
Culture in vitro
Alkaline phosphatase（AKP）
