摘要
为研究J-亚群禽白血病病毒(ALV-J)在安徽省鸡群中的遗传变异趋势,对分离到的2株安徽省ALV-J毒株,克隆了其gp85基因序列,并与国内外分离毒株的相应基因序列进行了比较。结果显示,ALVJ gp85基因序列具有高度的易变性,可变氨基酸残基占全长序列的22.8%。其中高变区hr1和hr2具有较多的可变位点,而可变区vr2和vr3相对较为保守。二级结构预测显示ALV-J gp85蛋白含有大量的无规则卷曲结构,尤其是hr2区域。同源性分析表明,毒株AH1103和AH1104的gp85氨基酸序列同源性为95.8%;毒株AH1103与毒株BJ090201J,ev/J的gp85序列同源性最高,毒株AH1104的gp85序列则与国内毒株HuB09JY04的同源性最高。进化分析显示,毒株AH1103和AH1104均与国内毒株NX0101和SD07LK1及美国分离株4817的遗传距离较远。
In order to further understand the genetic variation of subgroup J avian leukosis virus（ALV-J）in Anhui,ALV-J strains were isolated,and the gp85 gene was cloned by PCR.And we compared the amino acid sequences with thirteen sequences of ALV-J gp85 genes in the GenBank database.The results showed that ALV-J gp85 gene was highly variable,mutation sites accounted for 22.8% of full length amino acid sequence.hr1 and hr2 domains had high mutation frequency,while vr2 and vr3 domains were relatively more conservative.Protein secondary structure prediction showed ALV J gp85contained much random coil conformation,expecially hr2 domain.The homology between ALV-J gp85 amino acid sequences of strains AH1103 and AH1104 was 95.8%.The highest homology with strain AH1103 were strains BJ090201J and ev/J,and strain AH1104 had the highest homology with strain HuB09JY04isolated in China.Moreover,evolution analysis further revealed that the genetic distance strains AH1103 and AH1104 with strains NX0101,SD07LK1 and 4817 was farther.
出处
《动物医学进展》
CSCD
北大核心
2013年第12期38-41,共4页
Progress In Veterinary Medicine
基金
国家蛋鸡产业技术体系项目(CARS-41)
安徽省科技攻关项目(11010302119)
安徽省农业科学院科技创新团队基金项目(11C0404)
院长青年创新基金项目(11B0418)
