摘要
为评价J亚群禽白血病病毒(ALV-J)囊膜蛋白gp85基因作为ALV-J DNA疫苗的免疫原性,本研究通过PCR扩增ALV-J的gp85基因(930 bp),将其克隆于真核表达载体pVAX1中构建重组真核表达质粒pVAX1-gp85。间接免疫荧光检测结果显示gp85在转染的哺乳动物Hela细胞和鸡成纤维细胞均能够表达。将该重组质粒免疫雏鸡,利用ELISA试剂盒动态检测血清ALV-J抗体,结果显示pVAX1-gp85能够诱导大部分鸡只产生ALV-J抗体,并且抗体效价随加强免疫次数逐渐升高。本研究为开展ALV-J DNA疫苗的研究提供了实验依据。
To investigate the immunogenicity of the gp85 gene of subgroup J avian leukosis virus （ALV-J） as DNA vaccine, the 930 bp target gene was amplified from provirus genome of ALV-J and cloned into pVAX1 to construct the recombinant eukaryotic expressed plasmid pVAXl-gp85. Then, the test result showed that the ALV-J gp85 gene was transiently expressed in pVAXl-gp85 transfected Hela cells and CEF cells, respectively, which proved that the pVAXl-gp85 was able to efficiently express in mammalian and chicken cells in vitro. Moreover, the antibodies against ALV-J were detected in most chickens after 1 to 7 weeks post vaccination, and the titers increased with further booster immunizations. In conclusion, the ALV-J gp85 gene in pVAXl-gp85 was able to efficiently express and induce antibody in chickens against ALV-J.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2014年第9期727-730,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
公益性行业(农业)科研专项经费资助(201203055)
