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Dexamethasone down- regulates the activity of promoter from human a1(I) procollagen gene

Dexamethasone down- regulates the activity of promoter from human a1(I) procollagen gene
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摘要 Objective To investigate the effects of dexamethasone on the promoter activity of human a1(I) procollagen gene. Methods Fibroblasts from human skin were primary cultured and subcultured. (1)The effects of dexamethasone on the human skin fibroblasts were determined by BrdU incorporation into DNA of fibroblasts. (2)Three plasmids containing various lengths of 5’flank sequence of human a1(I) procollagen gene and CAT as reporter gene were constructed, and were transfected into the human skin fibroblasts by FuGENE Transfection Reagent. The effects of dexamethasone on 3 plasmids were determined by CAT- ELISA. Results (1)After 24h of treatment on the fibroblasts with 110- 9~ 110- 4mol/L dexamethasone in DMEM containing 2% or 10% FCS, BrdU incorporation into DNA showed no difference (P >0.05). (2) the 3 plasmids were transfected into fibroblasts and then treated with 110- 5mol/L and 110- 6mol/L dexamethasones for 24h, relative CAT values were different betwent dexamethasone and control, higher dexamethasone(110- 5mol/L) and lower dexamethasone(110- 6mol/L) (P< 0.05). Conclusion Dexamethasone has no effects on the proliferation of human skin fibroblasts, and it has negative effect on the promoter activity of human a1(I) procollagen gene, which is dose- dependent. Objective To investigate the effects of dexamethasone on the promoter activity of human a1(I) procollagen gene. Methods Fibroblasts from human skin were primary cultured and subcultured. (1)The effects of dexamethasone on the human skin fibroblasts were determined by BrdU incorporation into DNA of fibroblasts. (2)Three plasmids containing various lengths of 5'flank sequence of human a1(I) procollagen gene and CAT as reporter gene were constructed, and were transfected into the human skin fibroblasts by FuGENE Transfection Reagent. The effects of dexamethasone on 3 plasmids were determined by CAT- ELISA. Results (1)After 24h of treatment on the fibroblasts with 110- 9~ 110- 4mol/L dexamethasone in DMEM containing 2% or 10% FCS, BrdU incorporation into DNA showed no difference (P >0.05). (2) the 3 plasmids were transfected into fibroblasts and then treated with 110- 5mol/L and 110- 6mol/L dexamethasones for 24h, relative CAT values were different betwent dexamethasone and control, higher dexamethasone(110- 5mol/L) and lower dexamethasone(110- 6mol/L) (P< 0.05). Conclusion Dexamethasone has no effects on the proliferation of human skin fibroblasts, and it has negative effect on the promoter activity of human a1(I) procollagen gene, which is dose- dependent.
出处 《现代康复》 CSCD 2000年第12期1858-1859,共2页 Modern Rehabilitation
基金 Foundation item:This project was supported by a grant from national natural science foundation of china!(39870761) Biography:Wa
关键词 地塞料玄 胶原蛋白 基因 纤维变性 dexamethasone gene,collagen promotex
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