摘要
目的 :研究增生性瘢痕成纤维细胞中纤维化相关的转录因子。方法 :人皮肤增生性瘢痕成纤维细胞原代、传代培养后抽提核蛋白。人工法合成转录因子Sp 1、NF Ⅰ、NF κB识别序列的双链寡核苷酸 ,并采用Dig ddUTP 3′末端标记该双链寡核苷酸。电泳迁移率改变实验 (EMSA)法研究瘢痕成纤维细胞中纤维化相关的Sp 1、NF Ⅰ、NF κB等转录因子。结果 :在人增生性瘢痕成纤维细胞的核蛋白中存有纤维化相关的Sp 1、NF Ⅰ、NF κB等转录因子。结论 :瘢痕纤维化的发生、发展与纤维化相关的转录因子Sp 1、NF Ⅰ、NF κB密切相关。
Objective To analyze the transcription factors r elated to fibrosis in human hypertrophic scar fibroblasts. Methods Fibroblasts ob tained from the hypertrophic scar of a 3-year-old patient were primarily cultured and subcultu red in vitro, and then nucleoproteins were extracted from the cells. The double-st randed oligonucleotides specifically recognized by transcription factors Sp-1 , NF-Ⅰ, NF-κB were synthesized and end-labeled with Dig-ddUTP, then used a s a pr obe in electrophoretic mobility shift assays(EMSA) to identify the transcription factors related to fibrosis in hypertrophic scar fibroblasts. Result The data confirmed that there were transcription factors Sp-1, NF-Ⅰ, NF-κB in the hype rtrophic scar fibroblasts. Conclusion Hypertrophic scar develop ing is related to transcription factors Sp-1, NF-Ⅰ, NF-κB.
出处
《南京铁道医学院学报》
2004年第6期359-361,共3页
Journal of Nanjing Railway Medical College
基金
国家自然科学基金资助项目 (39870 761 )
